PMID: 9417779Jan 7, 1998Paper

A method to study kinetics of transnitrosation with nitrosoglutathione: reactions with hemoglobin and other thiols

Analytical Biochemistry
R RossiPaolo Di Simplicio

Abstract

The rate of protein S-nitrosylation, a reversible process by which S-nitroso thiol (RS-NO) compounds exchange the NO+ moiety with protein SH groups, is essentially governed by two factors, the pK alpha and the accessibility of the protein sulfhydryl. A useful method of following transnitrosation kinetics of various protein and nonprotein SH compounds with GS-NO is described. When the reaction is carried out in the presence of 1-chloro-2,4-dinitrobenzene and glutathione transferases, the rate of RS-NO formation (RSH + GS-NO-->RS-NO + GSH) can be monitored by spectrophotometry at 340 nm in terms of the enzymatic conversion of GSH to a GS conjugate. Unlike methods based on NO release from the S-NO bond, this procedure is rapid and accurate and requires relatively small amounts of thiols. The second order rate constants of S-nitrosylation of human and rat oxy- and deoxyhemoglobin of BSA and other thiols were calculated by this method which confirmed previous results reported in the literature.

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Apr 29, 1988·Biochemical and Biophysical Research Communications·J W Park
Jan 1, 1987·Methods in Enzymology·J HoukG M Whitesides
May 15, 1981·European Journal of Biochemistry·S G CondòM Brunori
Jan 1, 1981·Methods in Enzymology·A Riggs
Jan 1, 1981·Methods in Enzymology·P C Simons, D L Vander Jagt
Jan 1, 1995·Trends in Pharmacological Sciences·A R ButlerD L Williams
Mar 3, 1994·Nature·M FeelischS Moncada
Jan 1, 1994·Advances in Protein Chemistry·D C Carter, J X Ho
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B GastonD J Singel
Jun 1, 1996·Analytical Biochemistry·M S StudebakerG E Means
Sep 2, 1996·FEBS Letters·H WoloskerS Engelender
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

❮ Previous
Next ❯

Citations

Apr 4, 2012·Antioxidants & Redox Signaling·Katarzyna A Broniowska, Neil Hogg
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Neil Hogg
Jan 13, 2006·Nature Chemical Biology·Douglas A Mitchell, Michael A Marletta
Aug 5, 2008·Nitric Oxide : Biology and Chemistry·Daniela GiustariniRanieri Rossi
May 29, 2004·Trends in Pharmacological Sciences·Daniela GiustariniRanieri Rossi
May 5, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Raffaella PrioraPaolo Di Simplicio
Sep 12, 2013·Redox Biology·Cassilda PereiraJoão Laranjinha
Oct 6, 2000·Nitric Oxide : Biology and Chemistry·T IshibashiM Nishio
Feb 24, 2001·Bioorganic & Medicinal Chemistry Letters·K WangP G Wang
Mar 31, 2005·Archives of Biochemistry and Biophysics·Susanna Herold, Gabriele Röck
Oct 13, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniela GiustariniRanieri Rossi
Apr 21, 2006·Biochemical Pharmacology·Daniela GiustariniRanieri Rossi
Aug 16, 2003·The Journal of Biological Chemistry·Paula AracenaCecilia Hidalgo
Aug 7, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Steven DeemMark T Gladwin
Apr 11, 2013·The Journal of Biological Chemistry·David BalchinHeini W Dirr
Apr 5, 2000·The Journal of Biological Chemistry·T J McMahonJ S Stamler
May 21, 1999·The Journal of Biological Chemistry·R P PatelV M Darley-Usmar
Jan 13, 2005·The Journal of Biological Chemistry·Benjamin S RaynerPaul K Witting
Sep 12, 2015·The Journal of Toxicological Sciences·Yasuhiro ShinkaiYoshito Kumagai
Aug 18, 2000·The Journal of Biological Chemistry·N Y SpencerN Hogg
Mar 4, 2000·Free Radical Biology & Medicine·D Jourd'heuilM B Grisham
Apr 11, 2002·Chemical Reviews·Jonghyuk LeeGeorge B Richter-Addo
Jul 24, 1998·Archives of Biochemistry and Biophysics·P Di SimplicioR Rossi
Sep 16, 1999·Archives of Biochemistry and Biophysics·K TakakuraJ P Crow
Jan 26, 1999·Archives of Biochemistry and Biophysics·Y JiJ A Thomas
Mar 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Daniela GiustariniRanieri Rossi
Mar 21, 2003·Biochemical and Biophysical Research Communications·Rupam SahooSanjay Ghosh
Dec 4, 2003·Free Radical Biology & Medicine·Netanya Y SpencerNeil Hogg
Jul 18, 2001·Journal of the American Chemical Society·A A RomeoA M English
Jun 5, 2003·Journal of the American Chemical Society·K N HoukEric J Toone
Sep 1, 2001·The Journal of Organic Chemistry·X ChenP G Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.