A methodology for deriving the sensitivity of pooled testing, based on viral load progression and pooling dilution

Journal of Translational Medicine
Ngoc T NguyenDouglas R Bish

Abstract

Pooled testing, in which biological specimens from multiple subjects are combined into a testing pool and tested via a single test, is a common testing method for both surveillance and screening activities. The sensitivity of pooled testing for various pool sizes is an essential input for surveillance and screening optimization, including testing pool design. However, clinical data on test sensitivity values for different pool sizes are limited, and do not provide a functional relationship between test sensitivity and pool size. We develop a novel methodology to accurately compute the sensitivity of pooled testing, while accounting for viral load progression and pooling dilution. We demonstrate our methodology on the nucleic acid amplification testing (NAT) technology for the human immunodeficiency virus (HIV). Our methodology integrates mathematical models of viral load progression and pooling dilution to derive test sensitivity values for various pool sizes. This methodology derives the conditional test sensitivity, conditioned on the number of infected specimens in a pool, and uses the law of total probability, along with higher dimensional integrals, to derive pooled test sensitivity values. We also develop a highly accurat...Continue Reading

References

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Citations

May 3, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Idan YelinRoy Kishony
Jul 16, 2020·Diagnostics·Vlad PetrovanMihaela Zaulet
Apr 30, 2020·PharmacoEconomics Open·Koen B PouwelsPhilip M Clarke
Dec 4, 2020·Journal of Laboratory Physicians·Sangeeta Deka, Deepjyoti Kalita
Dec 9, 2020·BMC Medicine·David R M SmithUNKNOWN AP-HP/Universities/Inserm COVID-19 research collaboration
Feb 9, 2021·PloS One·Douglas R BishHrayer Aprahamian
Dec 31, 2020·Journal of Occupational and Environmental Medicine·Paul A SchulteJohn Howard
Sep 11, 2020·Disaster Medicine and Public Health Preparedness·Balram RaiAbhishek Singh
Sep 13, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Adolfo de SalazarFederico García

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Methods Mentioned

BETA
nucleic acid amplification
Assay

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