PMID: 1335Nov 1, 1975

A micro-method for quantitative determination of acylneuraminic acids from erythrocyte membranes

Hoppe-Seyler's Zeitschrift für physiologische Chemie
R SchauerD Danon

Abstract

A micro-method is presented which enables the fast and exact determination of acid-hydrolyzed acylneuraminic acids in erythrocyte membranes. Erythrocytes from 1 ml of human and rabbit blood containing ACD buffer are, washed and hemolyzed on Millipore filters of pore size 1.2 mu. Acylneuraminic acids are released from the erythrocyte membranes still on the filters under the optimal conditions of 0.1 N HCl at 80 degrees C for 50 min. A prerequisite for the determination of the true amount of acylneuraminic acids using the periodic acid/thiobarbituric acid assay is the small-scale extraction of lipids from the hydrolysate and anion-exchange chromatography of acylneuraminic acids. The values thus obtained must be corrected, as 20% of acylneuraminic acids are destroyed during acid hydrolysis. In samples of human blood from 10 healthy individuals, on an average 223 nmol acylneuraminic acids per ml of packed erythrocytes were found, and in the same amount of rabbit erythrocytes, 1e method for a screening of the acylneuraminic acid content of erythrocyte membranes in hemolytic diseases or of other cell membranes is discussed.

References

May 1, 1957·Biochimica Et Biophysica Acta·V S WARAVDEKAR, L D SASLAW

Citations

Feb 1, 1981·Experimental Parasitology·R J Howard, K P Day
Aug 1, 1995·Diabetic Medicine : a Journal of the British Diabetic Association·P J WatersR Eisenthal
Nov 1, 1992·The Journal of Clinical Investigation·R H YolkenD S Newburg
Nov 1, 1994·Immunological Investigations·J M GuthridgeD A Cohen
May 21, 1982·Journal of Theoretical Biology·R HeinrichR Glaser
Jun 1, 1981·The British Journal of Dermatology·H W Davies, M D Trotter
May 30, 1980·Biochemical and Biophysical Research Communications·G ReuterR J Howard
Nov 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·G Pfannschmidt, R Schauer
Mar 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A K Shukla, R Schauer

Related Concepts

Plasma Membrane
Erythrocytes
Hemolysis
Hydrogen-Ion Concentration
Hydrolysis
Sialic Acids

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