PMID: 6189849Feb 1, 1983Paper

A micro solid-phase radioimmunoassay for detection of herpesvirus type-specific antibody: specificity and sensitivity

Journal of Virological Methods
K Adler-StorthzG R Dreesman

Abstract

The specificity and sensitivity of a micro solid-phase radioimmunoassay (micro-SPRIA) that detects type-specific IgG antibody to herpes simplex virus types 1 and 2 (HSV1 and HSV2) were evaluated. Glycoproteins VP123 (molecular weight, 123,000) of HSV1 and VP119 (molecular weight, 119,000) of HSV2 were found to display the greatest degree of antigenic type-specificity of several HSV antigens tested with the micro-SPRIA technique. When testing a group of sera, negative for anti-HSV antibodies by microneutralization, in the micro-SPRIA, a range of negative reactivities was noted, suggesting that cut-points should be determined for each antigen preparation. The micro-SPRIA detected appropriate antibody activity in patients with recurrent infection and a marked agreement was noted in comparison to detection of anti-HSV antibodies measured with the microneutralization test. The type-specificity of the micro-SPRIA was substantiated by the independence of test results using VP119 and VP123 antigens for a random group of positive sera. The assay is rapid, specific, and sensitive and allows the testing of multiple serum samples with a standardized set of reagents.

References

Jan 1, 1979·Comparative Immunology, Microbiology and Infectious Diseases·I W SmithO G Gray
Jan 1, 1977·Acta Anaesthesiologica Scandinavica·J Viby-Mogensen, H K Hanel
Oct 1, 1972·The Journal of General Virology·T SavageJ W Heine
Jan 1, 1980·Journal of Immunological Methods·C Y Ching, C López

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Citations

Aug 1, 1986·Journal of Virological Methods·D KatzR A Word
Mar 1, 1985·American Journal of Obstetrics and Gynecology·K Adler-StorthzE Adam
Nov 5, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·F J NietoM Szklo
Jan 15, 1984·International Journal of Cancer. Journal International Du Cancer·V VonkaH Závadová

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