A microarray platform for detecting disease-specific circulating miRNA in human serum

Biosensors & Bioelectronics
Somenath RoyJackie Y Ying

Abstract

Circulating microRNAs (miRNAs) are emerging as potential blood-based biomarkers for cancer and other critical diseases. To profile the expression levels of these tiny molecules, especially in a point-of-care setting, it is imperative to quantify them directly in complex biological fluids. Herein, we report the development of a microarray platform with carboxyl-polyethylene glycol (PEG) as a functional layer and aminated hairpin nucleic acid molecules as target-specific capture probes (CPs). Due to the anti-fouling effect conferred by the carboxyl-PEG layer, we could directly detect as little as 10fM of miRNA targets in 20µl of unprocessed human serum. In contrast to the conventional miRNA microarrays, our platform does not require RNA extraction, labeling and target amplification, thus significantly reducing both the sample preparation steps as well as the total assay duration. The use of specially designed hairpin CPs entails reliable discrimination of miRNA sequences with high sequence homology. A nanoparticle-based detection technique, with the help of differential interference contrast (DIC) microscopy, offers excellent resolution down to a single molecule. With the capability of detecting disease-specific miRNA targets dir...Continue Reading

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Citations

Dec 6, 2016·Biomarkers in Medicine·Roberto Fiammengo
Mar 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gaia PalminiMaria Luisa Brandi
Dec 23, 2015·Chemical Society Reviews·Stacey LaingKaren Faulds
Sep 21, 2017·Luminescence : the Journal of Biological and Chemical Luminescence·Xianjiu LiaoQianli Tang
Aug 28, 2018·Journal of Cellular Physiology·Rupal OjhaVijay Kumar Prajapati
Nov 6, 2018·Analytical and Bioanalytical Chemistry·Catarina Coutinho, Álvaro Somoza
Mar 19, 2020·Sensors·Hsin-Yi WenChia-Chin Chiang
Oct 17, 2017·Analytical and Bioanalytical Chemistry·Despina P KalogianniTheodore K Christopoulos
Feb 23, 2020·Beilstein Journal of Nanotechnology·María Sanromán Iglesias, Marek Grzelczak
Apr 16, 2020·Trends in Analytical Chemistry : TRAC·Yu-Min WangWenwan Zhong
Feb 5, 2021·Chemical Society Reviews·Thomas JetValérie Taly
Jul 1, 2020·ACS Sensors·Yannick Rondelez, Guillaume Gines
Aug 25, 2021·Analytical Chemistry·Shaohua GongBo Tang
Feb 1, 2019·Analytical Chemistry·Tinglan OuyangQinyu Ge

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