Abstract
Circulating microRNAs (miRNAs) are emerging as potential blood-based biomarkers for cancer and other critical diseases. To profile the expression levels of these tiny molecules, especially in a point-of-care setting, it is imperative to quantify them directly in complex biological fluids. Herein, we report the development of a microarray platform with carboxyl-polyethylene glycol (PEG) as a functional layer and aminated hairpin nucleic acid molecules as target-specific capture probes (CPs). Due to the anti-fouling effect conferred by the carboxyl-PEG layer, we could directly detect as little as 10fM of miRNA targets in 20µl of unprocessed human serum. In contrast to the conventional miRNA microarrays, our platform does not require RNA extraction, labeling and target amplification, thus significantly reducing both the sample preparation steps as well as the total assay duration. The use of specially designed hairpin CPs entails reliable discrimination of miRNA sequences with high sequence homology. A nanoparticle-based detection technique, with the help of differential interference contrast (DIC) microscopy, offers excellent resolution down to a single molecule. With the capability of detecting disease-specific miRNA targets dir...Continue Reading
References
Feb 15, 1991·Science·S P FodorD Solas
Sep 1, 1993·Biomaterials·S SanoY Ikada
Feb 13, 2001·Nucleic Acids Research·P V RiccelliA S Benight
Jul 4, 2001·Biomaterials·Y G KoD J Ahn
May 7, 2002·Nature Reviews. Cancer·David Sidransky
Jan 28, 2004·Cell·David P Bartel
Sep 17, 2004·Nature·Victor Ambros
Jun 9, 2005·Nucleic Acids Research·Soon Jin OhKwan Yong Choi
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Apr 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Yu Lin ZhongKian Ping Loh
Sep 6, 2008·PloS One·Shlomit GiladAyelet Chajut
Jul 15, 2009·PloS One·Michael J LodesBrooke Anderson
Nov 10, 2010·PloS One·Hua ZhaoSong Liu
Feb 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Marissa WuJuewen Liu
Apr 29, 2011·Lab on a Chip·Somenath RoyZhiqiang Gao
Oct 8, 2011·PloS One·Akira WatahikiYuzhuo Wang
Feb 5, 2015·Small·Jue HouYanlin Song
Citations
Dec 6, 2016·Biomarkers in Medicine·Roberto Fiammengo
Jan 18, 2017·British Journal of Pharmacology·Lingling SiJinyong Peng
Mar 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gaia PalminiMaria Luisa Brandi
Apr 4, 2017·Sensors·Changbei MaJun Wang
Dec 23, 2015·Chemical Society Reviews·Stacey LaingKaren Faulds
Sep 21, 2017·Luminescence : the Journal of Biological and Chemical Luminescence·Xianjiu LiaoQianli Tang
Aug 28, 2018·Journal of Cellular Physiology·Rupal OjhaVijay Kumar Prajapati
Nov 6, 2018·Analytical and Bioanalytical Chemistry·Catarina Coutinho, Álvaro Somoza
Mar 19, 2020·Sensors·Hsin-Yi WenChia-Chin Chiang
Oct 17, 2017·Analytical and Bioanalytical Chemistry·Despina P KalogianniTheodore K Christopoulos
Jul 14, 2020·The Analyst·Luping FengHua Wang
Feb 23, 2020·Beilstein Journal of Nanotechnology·María Sanromán Iglesias, Marek Grzelczak
Sep 5, 2019·Nanoscale·Han DingPeifeng Li
Apr 16, 2020·Trends in Analytical Chemistry : TRAC·Yu-Min WangWenwan Zhong
Feb 5, 2021·Chemical Society Reviews·Thomas JetValérie Taly
Jan 25, 2021·Analytica Chimica Acta·Zhang ZhangGuoming Xie
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yue WuZhenxin Wang
Apr 23, 2021·International Journal of Biological Macromolecules·Anton M SolovjevIvan Yu Sakharov
Jul 22, 2021·Analytical Chemistry·Qiuxia HaoXiliang Luo
Jul 1, 2020·ACS Sensors·Yannick Rondelez, Guillaume Gines
Dec 31, 2019·Analytical Chemistry·Daxiu LiYun Xiang
Aug 25, 2021·Analytical Chemistry·Shaohua GongBo Tang
Jun 27, 2018·ACS Applied Materials & Interfaces·Luping FengHua Wang
Feb 1, 2019·Analytical Chemistry·Tinglan OuyangQinyu Ge