PMID: 6981740Jul 1, 1982Paper

A microassay for simultaneous measurement of in vivo rates of tryptophan hydroxylation and levels of serotonin in discrete brain nuclei

Journal of Neuroscience Methods
J B LongJ S Kizer

Abstract

A sensitive procedure for simultaneous determination of in vivo rates of tryptophan hydroxylation and levels of serotonin (5-HT) in discrete rat brain nuclei is described. Rates of tryptophan hydroxylation are estimated by 5-hydroxytryptophan (5-HTP) accumulation following l-aromatic amino acid decarboxylase inhibition by R04-4602/1. 5-HTP is separated from 5-HT by liquid cation exchange after which both 5-HTP and 5-HT are measured by a sensitive radioenzymatic assay. Following decarboxylase inhibition, 5-HTP accumulates over 30 min in 6 brain nuclei examined, with negligible levels of 5-HTP being measured in the absence of decarboxylase inhibition. 5-HT levels do not change significantly up to 45 min after decarboxylase inhibition. Comparison of rates of tryptophan hydroxylation determined in 12 different microdissected rat brain areas reveals a greater rate of 5-HT biosynthetic activity in raphe nuclei containing 5-HT cell bodies than in nuclei containing 5-HT terminals. Pretreatment with para-chlorophenylalanine markedly reduces both 5-HTP accumulation and 5-HT levels in the nucleus raphe dorsalis. With this procedure quantities as little as 10 pg of both 5-HTP and 5-HT can be measured, allowing estimation of in vivo seroton...Continue Reading

References

Jan 1, 1975·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·G Bartholini, A Pletscher
Nov 15, 1976·Life Sciences·L M Neckers, J L Meek
Oct 24, 1975·Brain Research·M J BrownsteinJ S Kizer
Nov 15, 1974·Brain Research·M PalkovitsJ M Saavedra
Aug 1, 1966·Journal of Neurochemistry·R J Baldessarini, I J Kopin

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Citations

Mar 18, 1983·European Journal of Pharmacology·J B LongJ S Kizer
Jan 1, 1993·Progress in Biophysics and Molecular Biology·M C Boadle-Biber
Feb 1, 1985·Pharmacology, Biochemistry, and Behavior·R A MuellerG R Breese
Jan 1, 1985·Neurochemistry International·F ChaouloffJ L Elghozi
Jan 1, 1991·Journal of Neurochemistry·M DiksicW Feindel
Mar 1, 1984·Journal of Neurochemistry·R G Knowles, C I Pogson
Jan 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M DiksicY L Yamamoto

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