A microbubble-sparged yeast propagation-fermentation process for bioethanol production

Biotechnology for Biofuels
Vijayendran RaghavendranJeffrey Green

Abstract

Industrial biotechnology will play an increasing role in creating a more sustainable global economy. For conventional aerobic bioprocesses supplying O2 can account for 15% of total production costs. Microbubbles (MBs) are micron-sized bubbles that are widely used in industry and medical imaging. Using a fluidic oscillator to generate energy-efficient MBs has the potential to decrease the costs associated with aeration. However, little is understood about the effect of MBs on microbial physiology. To address this gap, a laboratory-scale MB-based Saccharomyces cerevisiae Ethanol Red propagation-fermentation bioethanol process was developed and analysed. Aeration with MBs increased O2 transfer to the propagation cultures. Titres and yields of bioethanol in subsequent anaerobic fermentations were comparable for MB-propagated and conventional, regular bubble (RB)-propagated yeast. However, transcript profiling showed significant changes in gene expression in the MB-propagated yeast compared to those propagated using RB. These changes included up-regulation of genes required for ergosterol biosynthesis. Ergosterol contributes to ethanol tolerance, and so the performance of MB-propagated yeast in fed-batch fermentations sparged with 1...Continue Reading

References

Jul 12, 2002·Applied Biochemistry and Biotechnology·P HensirisakW H Velander
Nov 15, 2002·BMC Bioinformatics·Mark D RobinsonTimothy R Hughes
Jul 25, 2003·Applied Microbiology and Biotechnology·S AlfenoreS E Guillouet
Nov 5, 2003·Methods : a Companion to Methods in Enzymology·Gordon K Smyth, Terry Speed
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
May 16, 2006·Biochimica Et Biophysica Acta·Jerry KaplanCaroline C Philpott
Aug 28, 2007·Bioinformatics·Matthew E RitchieGordon K Smyth
May 16, 2008·FEMS Yeast Research·Olga de SmidtJacobus Albertyn
Feb 4, 2014·Genome Biology·Charity W LawGordon K Smyth
Mar 13, 2014·Applied and Environmental Microbiology·Clark M Henderson, David E Block
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Dec 4, 2015·Cold Spring Harbor Protocols·J Michael Cherry
Jul 10, 2016·BMC Research Notes·Kadriye InanCharles D Miller
Aug 11, 2016·Scientific Reports·Yanfei ChengXiuping He
May 19, 2017·Biotechnology for Biofuels·Zilong Qiu, Rongrong Jiang
Oct 24, 2017·Nucleic Acids Research·Ron CaspiPeter D Karp
Feb 6, 2018·Applied Microbiology and Biotechnology·Bruno Labate Vale da CostaAndreas Karoly Gombert
Mar 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Pierre Gélinas

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
electrophoresis
PCR
Hi-Seq
RNAseq

Software Mentioned

LIMMA
or
Funspec
TopHat2
Bioconductor
Thermo Xcalibur
Eve
Yeastract
R
HTSeq

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.