PMID: 489137Jan 1, 1979Paper

A microcytotoxicity assay using 51Cr for measuring specific cell-mediated and humoral immune responses

International Archives of Allergy and Applied Immunology
D Della PentaM E Phillips

Abstract

A microcytotoxicity assay (MCA) for measuring in vitro cell-mediated cytotoxicity (CMC) and humoral cytotoxicity which uses 51Cr-labelled target cells is described. Data is presented to show the uptake and rentention of 51Cr by various viable cultured cells. The CMC determined by 51Cr-labelled target cell retention in microtest II plate wells was verified by microscopic observation. Comparisons between the CMC observed 24 and 48 h after incubation of immune and normal lymphoid cells with 51Cr-labelled target cells were made. The factors, including savings in time and expense, which may make this modified MCA superior to other MCAs in use, are discussed.

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

International Journal of Cancer. Journal International Du Cancer
J P BrownI Hellström
The Journal of Immunology : Official Journal of the American Association of Immunologists
P B Blair, M A Lane
© 2021 Meta ULC. All rights reserved