A microfabricated porous collagen-based scaffold as prototype for skin substitutes

Biomedical Microdevices
Curtis D ChinSamuel K Sia

Abstract

An important element of artificial skin is a tissue scaffold that allows for fast host regeneration. We present a microfabrication strategy, based on gelling collagen-based components inside a microfluidic device, that produces well-controlled pore sizes inside the scaffold. This strategy can produce finely patterned tissue scaffolds of clinically relevant dimensions suitable for surgical handling. Compared to porous collagen-based sponges produced by lyophilization, microfabricated tissue scaffolds preserve the fibrous structure and ligand density of natural occurring collagen. A fibroblast migration assay revealed fast cellular migration through the pores, which is desired for rapid tissue ingrowth. Finally, we also demonstrate a strategy to use this microfabrication technique to build anatomically accurate, multi-component skin substitutes in a cost-effective manner.

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Citations

Jul 18, 2012·Bioprocess and Biosystems Engineering·Cristian A AcevedoJavier Enrione
May 18, 2013·Iranian Red Crescent Medical Journal·Mohsen HosseinkhaniMahnaz Abbasi
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Sep 20, 2011·Journal of Biomaterials Applications·Hojjat NaderiAhmad Reza Bahrami

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