A microfluidic approach to study the effect of mechanical stress on erythrocytes in sickle cell disease

Lab on a Chip
Maria Alejandra Lizarralde IragorriWassim El Nemer

Abstract

The human red blood cell is a biconcave disc of 6-8 × 2 μm that is highly elastic. This capacity to deform enables it to stretch while circulating through narrow capillaries to ensure its main function of gas exchange. Red cell shape and deformability are altered in membrane disorders because of defects in skeletal or membrane proteins affecting protein-protein interactions. Red cell properties are also altered in other pathologies such as sickle cell disease. Sickle cell disease is a genetic hereditary disorder caused by a single point mutation in the β-globin gene generating sickle haemoglobin (HbS). Hypoxia drives HbS polymerisation that is responsible for red cell sickling and reduced deformability. The main clinical features of sickle cell disease are vaso-occlusive crises and haemolytic anaemia. Foetal haemoglobin (HbF) inhibits HbS polymerisation and positively impacts red cell survival in the circulation but the mechanism through which it exerts this action is not fully characterized. In this study, we designed a microfluidic biochip mimicking the dimensions of human capillaries to measure the impact of repeated mechanical stress on the survival of red cells at the single cell scale under controlled pressure. We show th...Continue Reading

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Citations

Nov 24, 2018·British Journal of Haematology·Claudine LapoumeroulieMarie-Dominique Hardy-Dessources
Sep 12, 2019·Blood Advances·Pedro L MouraTimothy J Satchwell
May 20, 2020·Lab on a Chip·Yuncheng ManUmut A Gurkan
Sep 10, 2020·Frontiers in Physiology·Anna Bogdanova, Lars Kaestner
Aug 9, 2019·Blood Advances·Sara El HossValentine Brousse
May 23, 2019·Lab on a Chip·Francesca SorbaCristina Martin-Olmos
Mar 26, 2021·Frontiers in Molecular Biosciences·Anupam AichLucimara Gaziola de la Torre
Aug 29, 2020·Haematologica·Sara El HossWassim El Nemer

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Methods Mentioned

BETA
rheology
chip
flow cytometry
biochip

Software Mentioned

IDEAS
GraphPad Prism
DIVA

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