A microfluidic biochip for complete blood cell counts at the point-of-care

Technology
Umer HassanRashid Bashir

Abstract

Complete blood cell counts (CBCs) are one of the most commonly ordered and informative blood tests in hospitals. The results from a CBC, which typically include white blood cell (WBC) counts with differentials, red blood cell (RBC) counts, platelet counts and hemoglobin measurements, can have implications for the diagnosis and screening of hundreds of diseases and treatments. Bulky and expensive hematology analyzers are currently used as a gold standard for acquiring CBCs. For nearly all CBCs performed today, the patient must travel to either a hospital with a large laboratory or to a centralized lab testing facility. There is a tremendous need for an automated, portable point-of-care blood cell counter that could yield results in a matter of minutes from a drop of blood without any trained professionals to operate the instrument. We have developed microfluidic biochips capable of a partial CBC using only a drop of whole blood. Total leukocyte and their 3-part differential count are obtained from 10 μL of blood after on-chip lysing of the RBCs and counting of the leukocytes electrically using microfabricated platinum electrodes. For RBCs and platelets, 1 μL of whole blood is diluted with PBS on-chip and the cells are counted el...Continue Reading

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Nov 28, 2012·Lab on a Chip·Nima JokilaaksoRashid Bashir
Mar 21, 2013·Critical Reviews in Biomedical Engineering·Bashir MorshedTofy Mussivand
Dec 7, 2013·Science Translational Medicine·Nicholas N WatkinsRashid Bashir
Jun 6, 2014·Biomedical Microdevices·Umer Hassan, Rashid Bashir
Jun 24, 2014·Lab on a Chip·Zachary J SmithSebastian Wachsmann-Hogiu
Jan 30, 2015·Lab on a Chip·John NguyenYu Sun

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Citations

Dec 14, 2016·Analytical Chemistry·Samiksha NayakSamuel K Sia
Feb 7, 2019·Lab on a Chip·Taylor OeschgerDavid Erickson
Apr 25, 2019·Nature Biomedical Engineering·B ReddyR Bashir
Jul 6, 2019·Frontiers in Bioengineering and Biotechnology·Abhishek VembadiMohammad A Qasaimeh

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