A microfluidic chip containing a molecularly imprinted polymer and a DNA aptamer for voltammetric determination of carbofuran

Mikrochimica Acta
Shuhuai LiXionghui Ma

Abstract

An electrochemical microfluidic chip is described for the determination of the insecticide carbofuran. It is making use of a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. The analyte (carbofuran) is transported to the MIP and captured at the identification site in the channel. Then, carbofuran is eluted with carbinol-acetic acid and transported to the DNA aptamer on the testing position of the chip. It is captured again, this time by the aptamer, and detected by differential pulse voltammetry (DPV). The dual recognition (by aptamer and MIP) results in outstanding selectivity. Additionally, graphene oxide-supported gold nanoparticles (GO-AuNPs) were used to improve the sensitivity of electrochemical detector. DPV response is linear in the 0.2 to 50 nM carbofuran concentration range at a potential of -1.2 V, with a 67 pM detection limit. The method has attractive features such as its potential for high throughput, high degree of automation, and high integration. Conceivably, the method may be extended to other analytes for which appropriate MIPs and aptamers are available. Graphical abstract Schematic of an electrochemical microfluidic chip for carbofuran detection based on a molecularly imprinted ...Continue Reading

References

Sep 8, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·S Aswathy AromalDaizy Philip
Sep 5, 2015·Biosensors & Bioelectronics·Mariana Medina-SánchezArben Merkoçi
Mar 5, 2016·Chemical Society Reviews·Lingxin ChenJinhua Li
Mar 8, 2017·Analytical Chemistry·Shimaa Eissa, Mohammed Zourob

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Citations

Dec 3, 2020·Sensors·Kamonrat Phopin, Tanawut Tantimongcolwat
Feb 10, 2019·Biosensors & Bioelectronics·Mei LiuNongyue He
Aug 28, 2021·Journal of Hazardous Materials·Xin ZhangWeijun Kong

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