A microfluidic chip for screening individual cancer cells via eavesdropping on autophagy-inducing crosstalk in the stroma niche

Scientific Reports
Hacer Ezgi KarakasYoon-Kyoung Cho

Abstract

Autophagy is a cellular homeostatic mechanism where proteins and organelles are digested and recycled to provide an alternative source of building blocks and energy to cells. The role of autophagy in cancer microenvironment is still poorly understood. Here, we present a microfluidic system allowing monitoring of the crosstalk between single cells. We used this system to study how tumor cells induced autophagy in the stromal niche. Firstly, we could confirm that transforming growth factor β1 (TGFβ1) secreted from breast tumor cells is a paracrine mediator of tumor-stroma interaction leading to the activation of autophagy in the stroma component fibroblasts. Through proof of concept experiments using TGFβ1 as a model factor, we could demonstrate real time monitoring of autophagy induction in fibroblasts by single tumor cells. Retrieval of individual tumor cells from the microfluidic system and their subsequent genomic analysis was possible, allowing us to determine the nature of the factor mediating tumor-stroma interactions. Therefore, our microfluidic platform might be used as a promising tool for quantitative investigation of tumor-stroma interactions, especially for and high-throughput screening of paracrine factors that are ...Continue Reading

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Citations

Oct 12, 2018·Medicinal Research Reviews·Hendrik FolkertsValerie R Wiersma
Nov 18, 2020·Biotechnology and Bioengineering·Julian SchmitzAlexander Grünberger
Dec 9, 2020·Biosensors & Bioelectronics·Guocheng FangDayong Jin
Oct 30, 2020·Advanced Therapeutics·Pavithra SukumarMohammad A Qasaimeh
Dec 19, 2021·Journal of Gastrointestinal Cancer·Yunus Akkoc, Devrim Gozuacik

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Methods Mentioned

BETA
chip
MDA
transgenic
ELISA
chips
PCR

Software Mentioned

Imaris
COMSOL Multiphysics®
Image J

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