A microfluidic device for electrofusion of biological vesicles

Biomedical Microdevices
Guillaume Tresset, Shoji Takeuchi

Abstract

This paper reports a microfabricated device with high aspect-ratio electrodes and low power consumption for the electrofusion of liposomes and cells. The applications may range from gene transfection or cell tracking to biophysical studies of membrane proteins. The device consists of 250 microm thick silicon electrodes bonded to a glass substrate and covered by a PDMS-coated glass slide. Liposomes were first aligned by AC voltage at 300 kHz and then fused with short DC pulses. The fusion yield can reach 75% and is globally better for liposome diameters larger than 10 microm. The encapsulation of microbeads inside liposomes has also been demonstrated and opens up the route towards fusion-based delivery of artificial microstructures into cells.

Citations

May 18, 2007·Biomedical Microdevices·Yu-Jen Pan, Ruey-Jen Yang
Apr 15, 2009·Biomedical Microdevices·Andrew ClowBjörn Oback
Jan 6, 2009·Nature Methods·Alison M SkelleyJoel Voldman
Apr 21, 2009·PMC Biophysics·Guillaume Tresset
Jun 5, 2012·Biomicrofluidics·Shaoyan CuiYubo Fan
Jun 7, 2014·Chemical Reviews·Qingjun LiuPing Wang
Jun 21, 2014·Analytical Chemistry·Yuki YoshimuraTomoyuki Yasukawa
Aug 15, 2014·Journal of the Royal Society, Interface·Harold Fellermann, Luca Cardelli
Jul 12, 2014·Bioelectrochemistry·Feriel Siham HamdiBruno Le Pioufle
Feb 16, 2013·Chemical Reviews·Anthony S StenderNing Fang
Aug 11, 2010·Lab on a Chip·Tetsuhiko TeshimaShoji Takeuchi
Oct 14, 2014·International Journal of Molecular Sciences·Cheng QianLining Sun
Nov 17, 2009·Trends in Biotechnology·Séverine Le Gac, Albert van den Berg
Sep 5, 2015·Biomicrofluidics·Brett LittenPeter Fielden
Feb 6, 2015·Biomedical Microdevices·Srinivasu Valagerahally PuttaswamyYe Ai
May 30, 2013·Analytical Chemistry·Guillaume TressetCiprian Iliescu
Feb 26, 2016·Scientific Reports·Po-Fu YangGwo-Bin Lee
Jan 31, 2008·Lab on a Chip·Shia-Yen TehAbraham P Lee
Jun 2, 2006·Lab on a Chip·Wei-Heong Tan, Shoji Takeuchi
Jul 16, 2015·PloS One·Wei WuZheng Qin Yin
Aug 18, 2012·Electrophoresis·Guillaume MottetLluis M Mir
Nov 13, 2012·RSC Advances·Yevgeniy V KalininDavid H Gracias
Nov 8, 2018·Biomedical Microdevices·Mohd Anuar Md AliBurhanuddin Yeop Majlis
Jan 2, 2020·Biomicrofluidics·Weihua HeWenhui Wang
Feb 9, 2018·Engineering in Life Sciences·Phillip KuhnPetra S Dittrich
Aug 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ebrahim M Kolahdouz, David Salac
May 28, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Jonathan WestPetra S Dittrich
Feb 4, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Julian ThieleStephan Förster
Dec 8, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Takeshi SunamiSatoshi Fujii

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.