A microfluidic device for multiplexed protein detection in nano-liter volumes.

Analytical Biochemistry
Alan H DiercksAlan Aderem

Abstract

We describe a microfluidic immunoassay device that permits sensitive and quantitative multiplexed protein measurements on nano-liter-scale samples. The device exploits the combined power of integrated microfluidics and optically encoded microspheres to create an array of approximately 100-microm(2) sensors functionalized with capture antibodies directed against distinct targets. This strategy overcomes the need for performing biochemical coupling of affinity reagents to the device substrate, permits multiple proteins to be detected in a nano-liter-scale sample, is scalable to large numbers of samples, and has the required sensitivity to measure the abundance of proteins derived from single mammalian cells. The sensitivity of the device is sufficient to detect 1000 copies of tumor necrosis factor (TNF) in a volume of 4.7nl.

References

Oct 27, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·X RenN Allbritton
Jun 19, 2002·Analytical Chemistry·Anne Y FuStephen R Quake
Jul 18, 2002·Nature Reviews. Drug Discovery·Kevin BraeckmansJoseph Demeester
Sep 28, 2002·Science·Todd ThorsenStephen R Quake
Sep 17, 2003·Genome Research·Matthew O ReeseStephen R Quake
Oct 7, 2004·Analytical Biochemistry·Yoshihiro MurakamiEiichi Tamiya
Oct 19, 2004·Analytical Chemistry·Nancy H FinkelLin He
Apr 28, 2005·Biosensors & Bioelectronics·Adam BangeWilliam R Heineman
Jun 28, 2005·Trends in Biotechnology·Richard P AuburnSteven Russell
Jul 11, 2006·Advanced Drug Delivery Reviews·Kim L Wark, Peter J Hudson

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Citations

Apr 28, 2010·Analytical and Bioanalytical Chemistry·Alphonsus H C NgAaron R Wheeler
Jun 16, 2010·Analytical and Bioanalytical Chemistry·Iuliana OitaDebby Mangelings
Mar 26, 2010·Analytical Chemistry·Weishan LiuRustem F Ismagilov
Mar 5, 2013·ACS Applied Materials & Interfaces·Chan-Hee JungYoung-Moo Lee
Mar 29, 2011·Nature Nanotechnology·J L ArlettM L Roukes
Jan 21, 2011·Journal of the Royal Society, Interface·Nora TheilackerXiaohua Huang
May 27, 2011·Journal of Biological Engineering·Mandy Ly SinPak Kin Wong
Jan 1, 2009·Sensors·Jürgen MairhoferPeter Ertl
Mar 25, 2014·Annual Review of Immunology·Daniel E ZakAlan Aderem
Aug 13, 2014·BioMed Research International·Cihun-Siyong Alex Gong, Kin Fong Lei
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Janine MokMehdi Javanmard
Mar 29, 2011·Current Opinion in Biotechnology·Marcus TauppSteven J Hallam
Feb 6, 2013·IEEE Transactions on Bio-medical Engineering·John P WikswoStacy D Sherrod
Jul 15, 2015·Bioanalysis·Cameron D YamanishiShuichi Takayama
Oct 30, 2016·Molecular and Cellular Probes·Valentina BusinStewart T G Burgess
May 12, 2017·Chemistry, an Asian Journal·Lu HuangHongkai Wu
Dec 2, 2015·Lab on a Chip·Duane S Juang, Chia-Hsien Hsu
Aug 16, 2012·Journal of Laboratory Automation·Kin Fong Lei
Nov 7, 2018·Lab on a Chip·Aude I SegalinyWeian Zhao
Aug 8, 2009·Angewandte Chemie·Chuanbin MaoBinrui Cao
Nov 24, 2011·Optics Express·C P K MancheeA Meldrum
Jul 6, 2013·Lab on a Chip·John P WikswoWilliam J Matloff
Oct 1, 2012·Microfluidics and Nanofluidics·Lawrence A SassoJeffrey D Zahn
Feb 14, 2017·Nano Convergence·Inhong KimJin-Woo Oh
Nov 22, 2018·Frontiers in Immunology·Nidhi SinhaJurjen Tel
Aug 8, 2021·Nanomaterials·Hiromi TakahashiYoshinobu Baba
Dec 28, 2017·ACS Applied Materials & Interfaces·Richard WollhofenJaroslaw Jacak

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