A microfluidic device for noninvasive cell electrical stimulation and extracellular field potential analysis

Biomedical Microdevices
Liwei NiJiang Zhe

Abstract

We developed a device that can quickly apply versatile electrical stimulation (ES) signals to cells suspended in microfluidic channels and measure extracellular field potential simultaneously. The device could trap cells onto the surface of measurement electrodes for ES and push them to the downstream channel after ES by increasing pressure for continuous measurement. Cardiomyocytes, major functional cells in heart, together with human fibroblast cells and human umbilical vein endothelial cells, were tested with the device. Extracellular field potential signals generated from the cells were recorded. We found that under electrical stimulation, cardiomyocytes were triggered to alter their field potential, while non-excitable cells were not triggered. Hence this device can noninvasively distinguish electrically excitable cells from electrically non-excitable cells. Results have also shown that increased cardiomyocyte cell number led to increased magnitude and occurrence of the cell responses. This relationship could be used to detect the viable cells in a cardiac tissue. Application of variable ES signals on different cardiomyocyte clusters has shown that the application of ES clearly boosted cardiomyocytes electrical activities ...Continue Reading

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Citations

Feb 23, 2020·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Liudmila LeppikJohn Howard Barker
Jan 12, 2021·Microfluidics and Nanofluidics·Adem Ozcelik, Zeynep Aslan

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