A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans.

Lab on a Chip
Shawn R LockeryJanis C Weeks

Abstract

This paper describes the fabrication and use of a microfluidic device for performing whole-animal chemical screens using non-invasive electrophysiological readouts of neuromuscular function in the nematode worm, C. elegans. The device consists of an array of microchannels to which electrodes are attached to form recording modules capable of detecting the electrical activity of the pharynx, a heart-like neuromuscular organ involved in feeding. The array is coupled to a tree-like arrangement of distribution channels that automatically delivers one nematode to each recording module. The same channels are then used to perfuse the recording modules with test solutions while recording the electropharyngeogram (EPG) from each worm with sufficient sensitivity to detect each pharyngeal contraction. The device accurately reported the acute effects of known anthelmintics (anti-nematode drugs) and also correctly distinguished a specific drug-resistant mutant strain of C. elegans from wild type. The approach described here is readily adaptable to parasitic species for the identification of novel anthelmintics. It is also applicable in toxicology and drug discovery programs for human metabolic and degenerative diseases for which C. elegans i...Continue Reading

References

Jul 1, 1987·The Journal of Physiology·W M Roberts
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·J A DentL Avery
Mar 24, 2000·Science·G M Rubin, E B Lewis
Mar 10, 2004·Journal of Biomolecular Screening·Brenda R EllerbrockTimothy G Geary
Jan 5, 2005·Nature Reviews. Genetics·Ethan Bier
Apr 4, 2006·Genetics·M A ToroL Silió
May 5, 2006·Nature Reviews. Drug Discovery·Titus Kaletta, Michael O Hengartner
Apr 19, 2007·Nature Reviews. Genetics·Graham J Lieschke, Peter D Currie
May 15, 2007·Clinical Pharmacology and Therapeutics·G KariA P Dicker
Aug 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christopher B RohdeMehmet Fatih Yanik
Oct 26, 2007·Lab on a Chip·S Elizabeth HulmeGeorge M Whitesides
May 30, 2008·Nature·Nicolas RochFranck Balestro
Jun 10, 2008·Neurotoxicology·Randall T PetersonToshio Narahashi
Jun 24, 2008·Nature Methods·Kwanghun ChungHang Lu
Jul 22, 2008·Methods in Molecular Biology·Rachel Drysdale, UNKNOWN FlyBase Consortium
Aug 9, 2008·Invertebrate Neuroscience : in·Steven D Buckingham, David B Sattelle
Jan 27, 2009·Neurotoxicology and Teratology·Windy A BoydJonathan H Freedman
Feb 18, 2010·Lab on a Chip·S Elizabeth HulmeGeorge M Whitesides
Apr 29, 2010·British Journal of Pharmacology·Jean Giacomotto, Laurent Ségalat
May 7, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Donald WlodkowicJonathan M Cooper
Jul 16, 2011·Annual Review of Biomedical Engineering·Mehmet Fatih YanikCarlos Pardo-Martin

❮ Previous
Next ❯

Citations

Nov 13, 2012·Analytical Chemistry·Michelle L KovarikNancy L Allbritton
Feb 22, 2013·Chemical Reviews·Venkataragavalu Sivagnanam, Martin A M Gijs
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Johannes LarschDirk R Albrecht
Jul 26, 2013·Lab on a Chip·Thanh D NguyenMichael C McAlpine
Feb 10, 2016·Molecular Neurodegeneration·Matteo CornagliaMartin A M Gijs
Jan 17, 2016·Journal of Proteomics·Carlos Sánchez-OvejeroRaúl Manzano-Román
Sep 3, 2013·Current Opinion in Pharmacology·Marios ArvanitisEleftherios Mylonakis
May 6, 2015·The Journal of Nutritional Biochemistry·Chun-Hao HuangShyh-Horng Chiou
Dec 21, 2012·European Journal of Immunology·Andrea CossarizzaAttila Tárnok
May 27, 2015·Lab on a Chip·Jinzhou YuanHaim H Bau
Mar 7, 2015·Biomedical Microdevices·Pengfei SongXinyu Liu
Jan 15, 2015·The Journal of Parasitology·Timothy G GearyConor R Caffrey
Mar 17, 2015·Advances in Colloid and Interface Science·L Gonzalez-MoragasA Laromaine
Jul 31, 2016·Journal of Neuroscience Methods·Monika ScholzDavid Biron
Aug 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muniesh Muthaiyan Shanmugam, Tuhin Subhra Santra
Oct 19, 2016·International Journal for Parasitology. Drugs and Drug Resistance·Janis C WeeksJohn M Hawdon
Jun 13, 2018·Annual Review of Analytical Chemistry·Farhan Kamili, Hang Lu
Jul 11, 2018·Lab on a Chip·Krishna N BadhiwalaJacob T Robinson
Aug 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Monika ScholzDavid Biron
Feb 28, 2018·Scientific Reports·Mª Jesús Rodríguez-PaleroMarta Artal-Sanz
Nov 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniel Midkiff, Adriana San-Miguel
Apr 19, 2017·Nature Nanotechnology·Daniel L GonzalesJacob T Robinson
Jul 19, 2016·Micromachines·Bhagwati P Gupta, Pouya Rezai
Jun 30, 2019·Integrative Biology : Quantitative Biosciences From Nano to Macro·Khaled YoussefPouya Rezai
Dec 11, 2020·The Science of the Total Environment·Yutao BaiDonald Wlodkowic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.