A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis

Analytica Chimica Acta
Xiaoyan PanJianhua Qin

Abstract

This work describes a microfluidic device integrated with multichamber polymerase chain reaction (PCR) and multichannel separation for parallel genetic analysis. The microdevice consists of three functional units: temperature control, multiple PCR (four chambers PCR), and multiple channel separation (four separation channels, each channel connected to a PCR chamber). Platinum (Pt)/titanium (Ti) microheater was used to ensure homogeneous temperature field, and Pt-chip sensor was used for temperature monitoring. The interface between chip-PCR and chip separation was simplified by connecting the PCR chamber with separation channel directly. After chip-PCR, PCR products were introduced into parallel separation channels for subsequent separation/detection by applying an electric field automatically. This microdevice was successfully applied for detection of pathogens including hepatitis B virus (HBV) and Mycobacterium tuberculosis (MTB), and genotyping of human leucocyte antigen (HLA)-B27 as well, demonstrating the feasibility of the integrated microdevice for parallel genetic analysis.

References

Dec 30, 1998·Analytical Chemistry·L C WatersJ M Ramsey
Apr 26, 2003·Analytical Chemistry·Michael C BreadmoreJames P Landers
Apr 22, 2004·Lab on a Chip·E T LagallyR A Mathies
May 26, 2005·Lab on a Chip·James H AbornDaniel J Ehrlich
Jun 15, 2005·Analytical Chemistry·Michael G RoperJames P Landers
Feb 14, 2006·Electrophoresis·Zheng ShenBingcheng Lin
May 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert G BlazejRichard A Mathies
Aug 2, 2006·Analytical Chemistry·Chung N LiuRichard A Mathies
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christopher J EasleyJames P Landers
Aug 29, 2007·Analytica Chimica Acta·Thomas Ming-Hung Lee, I-Ming Hsing
Oct 26, 2007·Lab on a Chip·Lin ChenPhilip J R Day

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Citations

May 18, 2013·Analytical and Bioanalytical Chemistry·Nongnoot WongkaewAntje J Baeumner
Feb 18, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fei FanXixiong Kang
Aug 17, 2012·Medical Physics·Tian-Cai LiuLi-Ping Wang
Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Dong-Sheng Lian, Shu-Jin Zhao
Dec 1, 2011·Electrophoresis·Jinwen ZhouNicolas H Voelcker
Sep 25, 2014·Lab on a Chip·Delphine Le RouxPhilippe de Mazancourt

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