A microfluidic device that forms and redirects pheromone gradients to study chemotropism in yeast

Lab on a Chip
Marie-Elena BrettDavid T Eddington

Abstract

Chemotropism, or directed cell growth in response to a chemical gradient, is integral to many biological processes. The mating response of the budding yeast, Saccharomyces cerevisiae, is a well studied model chemotropic system. Yeast cells of opposite mating type signal their positions by secreting soluble mating pheromones. The mutual exchange of pheromones induces the cells to grow towards one another, resulting in mating projections or "shmoos." Yeast cells exhibit a remarkable ability to orient their growth toward the nearest potential mating partner, and to reorient (i.e., bend their mating projections) in response to a change in the direction of the pheromone gradient. Although a number of microfluidic devices have been used to generate linear pheromone gradients and to measure initial orientation, none of them have the capability to change the direction of the gradient, other than to invert it. We have developed a microfluidic device that can produce stable pheromone gradients and rapidly rotate them in 90° increments, mimicking the dynamic gradients yeast are exposed to in situ, and allowing for the study of reorientation as well as initial orientation. The mean angle of orientation exhibited by gradient-stimulated yeas...Continue Reading

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Citations

Nov 13, 2012·Analytical Chemistry·Michelle L KovarikNancy L Allbritton
Jan 12, 2013·Current Biology : CB·Robert A Arkowitz
Oct 8, 2016·Small·Myeong Chan Jo, Lidong Qin
Apr 7, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Marie-Elena BrettDavid K Wood
May 3, 2013·Physical Chemistry Chemical Physics : PCCP·Matthew D EstesFrederic Zenhausern
Nov 24, 2020·Frontiers in Microbiology·Carolin SchunkeDaniela Elisabeth Nordzieke
Mar 17, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Khanh L LyXiaolong Luo
Apr 25, 2013·Journal of Cell Science·Reagan DeflorioDavid E Stone
Mar 11, 2021·Molecular Biology of the Cell·Manuella R Clark-CottonDaniel J Lew

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