A microfluidic flow distributor generating stepwise concentrations for high-throughput biochemical processing

Lab on a Chip
Masumi YamadaMinoru Seki


In this paper, we describe a microfluidic device in which solutions with stepwise concentrations can be accurately generated by continuously introducing two kinds of miscible liquids from each inlet, and biochemical processing can be conducted at the various conditions. Introduced liquid flows are geometrically divided into a number of downstream flows through multiple distribution channels, and each divided flow is then mixed with the divided flow of another liquid at a confluent point. The lengths of the precisely designed distribution channels determine the mixing ratio of the two liquids, without the influence of flow rate. In this study, a PDMS microfluidic device able to generate nine different concentrations was fabricated, and the performance of this device was estimated via colorimetric assay. As a biological application of this device, cell cultivation was performed under different concentration conditions. Due to its simplicity of operation, this microfluidic flow distributor will be applied to various kinds of biological analysis and screening systems.


Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D T ChiuG M Whitesides
Jan 29, 2002·Analytical Chemistry·Hanbin MaoPaul S Cremer
Apr 19, 2002·Journal of the American Chemical Society·Hanbin MaoPaul S Cremer
Oct 17, 2002·Analytical Chemistry·Hanbin MaoPaul S Cremer
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carl L HansenStephen R Quake
May 2, 2003·Journal of the American Chemical Society·Xingyu JiangGeorge M Whitesides
Apr 8, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nae Yoon LeeMinoru Seki
Apr 22, 2004·Lab on a Chip·Yoshikuni KikutaniTakehiko Kitamori
Jul 23, 2004·Lab on a Chip·Christopher NeilsAlbert Folch
Dec 18, 2004·Biomaterials·Shinji SugiuraMitsutoshi Nakajima
Jul 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Jason A BurdickRobert Langer
Jul 1, 2005·Analytical Chemistry·Johan PihlOwe Orwar
Oct 14, 2005·Journal of the American Chemical Society·Bo ZhengRustem F Ismagilov

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Mar 22, 2008·Analytical Chemistry·Małgorzata A WitekSteven A Soper
Jan 8, 2014·Biomicrofluidics·Shan-Wen HuHong-Yuan Chen
Sep 27, 2008·Lab on a Chip·Kang SunXingyu Jiang
Jun 17, 2008·Biotechnology and Bioengineering·Shinji SugiuraToshiyuki Kanamori
Nov 17, 2007·Electrophoresis·Thomas FriskHjalmar Brismar
May 23, 2015·Lab on a Chip·Jinzhen FanTingrui Pan
Feb 3, 2007·Lab on a Chip·Glenn M WalkerAdrian T O'Neill
Dec 20, 2011·Lab on a Chip·Kwang W OhEdward P Furlani
Aug 3, 2014·Journal of Bioscience and Bioengineering·Kazunori ShimizuSatoshi Konishi
May 24, 2008·Analytical Chemistry·Jonathan WestAndreas Manz

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