A microfluidic flow distributor generating stepwise concentrations for high-throughput biochemical processing

Lab on a Chip
Masumi YamadaMinoru Seki

Abstract

In this paper, we describe a microfluidic device in which solutions with stepwise concentrations can be accurately generated by continuously introducing two kinds of miscible liquids from each inlet, and biochemical processing can be conducted at the various conditions. Introduced liquid flows are geometrically divided into a number of downstream flows through multiple distribution channels, and each divided flow is then mixed with the divided flow of another liquid at a confluent point. The lengths of the precisely designed distribution channels determine the mixing ratio of the two liquids, without the influence of flow rate. In this study, a PDMS microfluidic device able to generate nine different concentrations was fabricated, and the performance of this device was estimated via colorimetric assay. As a biological application of this device, cell cultivation was performed under different concentration conditions. Due to its simplicity of operation, this microfluidic flow distributor will be applied to various kinds of biological analysis and screening systems.

References

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Citations

Mar 22, 2008·Analytical Chemistry·Małgorzata A WitekSteven A Soper
Jan 8, 2014·Biomicrofluidics·Shan-Wen HuHong-Yuan Chen
Sep 27, 2008·Lab on a Chip·Kang SunXingyu Jiang
Jun 17, 2008·Biotechnology and Bioengineering·Shinji SugiuraToshiyuki Kanamori
Nov 17, 2007·Electrophoresis·Thomas FriskHjalmar Brismar
May 23, 2015·Lab on a Chip·Jinzhen FanTingrui Pan
Feb 3, 2007·Lab on a Chip·Glenn M WalkerAdrian T O'Neill
Dec 20, 2011·Lab on a Chip·Kwang W OhEdward P Furlani
Aug 3, 2014·Journal of Bioscience and Bioengineering·Kazunori ShimizuSatoshi Konishi
May 24, 2008·Analytical Chemistry·Jonathan WestAndreas Manz

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