A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment

ACS Synthetic Biology
Chung Sze LukeJeff Hasty

Abstract

Culturing cells in microfluidic "lab-on-a-chip" devices for time lapse microscopy has become a valuable tool for studying the dynamics of biological systems. Although microfluidic technology has been applied to culturing and monitoring a diverse range of bacterial and eukaryotic species, cyanobacteria and eukaryotic microalgae present several challenges that have made them difficult to culture in a microfluidic setting. Here, we present a customizable device for the long-term culturing and imaging of three well characterized strains of cyanobacteria and microalgae. This platform has several advantages over agarose pads and demonstrates great potential for obtaining high quality, single-cell gene expression data of cyanobacteria and algae in precisely controlled, dynamic environments over long time periods.

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Citations

May 18, 2016·Journal of Biotechnology·Kiran S DharKesavan Madhavan Nampoothiri
Sep 10, 2016·Scientific Reports·Kenneth K Y HoAllen P Liu
Dec 24, 2018·Physiologia Plantarum·Giorgio PerinTomas Morosinotto
Oct 1, 2019·Expert Review of Molecular Diagnostics·Kitti IntuyodSomchai Pinlaor
Mar 24, 2020·Nature Microbiology·Kristin A MooreJeffrey C Cameron
Mar 1, 2019·Biotechnology for Biofuels·Pierre BodénèsChun-Yen Wang
Nov 28, 2020·Analytical and Bioanalytical Chemistry·Fangchen LiuMingming Wu
Jan 8, 2021·Biotechnology and Bioengineering·Berin OzdalgicSavas Tasoglu
Mar 20, 2021·Biotechnology and Bioengineering·Thijn VerburgYe Wang
Feb 22, 2018·Analytical Chemistry·Mathias GiraultJean-Christophe Baret
Mar 9, 2018·ACS Synthetic Biology·Mathieu C HusserSteve C C Shih

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