A microfluidic system for high-speed reproducible DNA sizing and quantitation

Electrophoresis
Odilo MuellerD Ilsley

Abstract

Microfabrication technology was used to develop a system consisting of disposable glass chips containing etched channels, reagents including polymer matrix and size standards, computer-controlled instrumentation for performing electrophoretic separations and fluorescence detection of double-stranded DNA, and software for automated data analysis. System performance was validated for separation and quantitation reproducibility using samples varying in amount and size of DNA fragments, buffer composition, and salt concentrations. Several applications of the microfluidic system for DNA analysis have been demonstrated, such as of polymerase chain reaction (PCR) products, sizing of plasmid digests, and detection of point mutations by restriction fragment length polymorphism (RFLP) mapping.

References

Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·A T Woolley, R A Mathies
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·Mark A BurnsD T Burke
Oct 15, 1995·Analytical Chemistry·A T Woolley, R A Mathies
Jul 15, 1997·Analytical Chemistry·R M McCormickH H Hooper

Citations

Mar 14, 2002·Electrophoresis·Elisabeth Verpoorte
Apr 20, 2001·Luminescence : the Journal of Biological and Chemical Luminescence·J Ferrance, J P Landers
May 26, 2001·Journal of Colloid and Interface Science·Kevin D. Dorfman, Howard Brenner
May 18, 2011·International Journal of Legal Medicine·Antje KoppelkammMarielle Vennemann
Dec 25, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Truls Wergeland HansenØystein Sæle
Apr 24, 2013·Gene·Anna MinariniCarmela Fimognari
Dec 4, 2003·Neuroscience Letters·Annamaria ConfaloniAlfredo Cantafora
Apr 16, 2003·Analytical Biochemistry·Li DingRobert Dubrow
Mar 12, 2003·Advanced Drug Delivery Reviews·Bernhard H WeiglCatherine R Cabrera
May 12, 2012·The Journal of Physical Chemistry. B·Perry G SchiroDaniel T Chiu
Oct 10, 2012·Journal of Experimental Botany·Jose V Die, Belén Román
Nov 21, 2012·Nucleic Acids Research·Luca La ViaAlessandro Barbon
Nov 7, 2003·Nucleic Acids Research·Stephan LaibStefan Seeger
Jan 5, 2007·Journal of Clinical Microbiology·Lucy J HathawayKathrin Mühlemann
Jul 24, 2010·Canadian Journal of Microbiology·Ravi Ramesh Pathak, Sunila Lochab
Mar 15, 2005·BMC Genomics·Sherry F GrissomCharles J Tucker
Feb 2, 2006·BMC Molecular Biology·Andreas SchroederThomas Ragg
Jul 29, 2010·BMC Research Notes·Lydia V RumpNarjol Gonzalez-Escalona
Jan 1, 2009·Plant Molecular Biology Reporter·Michael A PhillipsJonathan Gershenzon
Dec 20, 2005·Expert Review of Molecular Diagnostics·Maria I Handal, Victor M Ugaz
Aug 21, 2010·Forensic Science International : Synergy·Marielle Vennemann, Antje Koppelkamm
Jul 14, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Øystein SaeleKristin Hamre
Jul 21, 2009·Journal of Pharmaceutical and Biomedical Analysis·Rie AkamineMasatoshi Kataoka
Oct 30, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Øystein SaelePål A Olsvik
Aug 5, 2008·Journal of Microbiological Methods·Courtney E JahnDavid K Willis
Dec 1, 2008·Meat Science·L FontanesiV Russo
Dec 7, 2007·Journal of Microbiological Methods·Joakim BjerketorpJanet K Jansson
Dec 19, 2006·Experimental and Molecular Pathology·Neera V Gopee, Paul C Howard
Feb 11, 2005·Electrophoresis·Alok SrivastavaGeorge E Georghiou
Jun 8, 2007·Journal of Forensic Sciences·Katie M HorsmanJames P Landers
Apr 18, 2013·Chembiochem : a European Journal of Chemical Biology·Pui Sai LauYingfu Li
Sep 5, 2006·International Journal of Hygiene and Environmental Health·T CartonM Joyeux
Feb 14, 2006·Molecular Aspects of Medicine·Simone Fleige, Michael W Pfaffl
May 17, 2005·Brain Research. Molecular Brain Research·Annamaria ConfaloniGianluigi Forloni
Sep 6, 2011·Analytical Biochemistry·Jose V DieBelén Román
Apr 16, 2016·Biopreservation and Biobanking·Guangwen ZhangDa Pang
May 31, 2014·Free Radical Biology & Medicine·Kristin HamrePål A Olsvik
Oct 27, 2011·The Journal of Molecular Diagnostics : JMD·Alberto ZamòAldo Scarpa
Oct 9, 2008·Mutation Research·Carmela FimognariPatrizia Hrelia
Jun 7, 2014·FEMS Yeast Research·Ran ChaoHuimin Zhao
May 14, 2016·Scandinavian Journal of Clinical and Laboratory Investigation·Lea LandoltOystein Solberg Eikrem
Apr 14, 2016·BioTechniques·Jessica DaviesJames Hadfield
Aug 17, 2016·Biosensors·Brigitte BruijnsHan Gardeniers
Jul 21, 2012·The British Journal of Nutrition·Øystein SæleKristin Hamre
Aug 13, 2004·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Victor M UgazMark A Burns
Nov 6, 2015·BMC Research Notes·Takahiro OguraShuichi Mizuno
Jun 25, 2017·Scientific Reports·Zhi-Gang ShenHan-Ping Wang

Related Concepts

Diffusion
DNA, Double-Stranded
Electrophoresis

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.