A microfluidic system for large DNA molecule arrays

Analytical Chemistry
Eileen T DimalantaD C Schwartz

Abstract

Single molecule approaches offer the promise of large, exquisitely miniature ensembles for the generation of equally large data sets. Although microfluidic devices have previously been designed to manipulate single DNA molecules, many of the functionalities they embody are not applicable to very large DNA molecules, normally extracted from cells. Importantly, such microfluidic devices must work within an integrated system to enable high-throughput biological or biochemical analysis-a key measure of any device aimed at the chemical/biological interface and required if large data sets are to be created for subsequent analysis. The challenge here was to design an integrated microfluidic device to control the deposition or elongation of large DNA molecules (up to millimeters in length), which would serve as a general platform for biological/biochemical analysis to function within an integrated system that included massively parallel data collection and analysis. The approach we took was to use replica molding to construct silastic devices to consistently deposit oriented, elongated DNA molecules onto charged surfaces, creating massive single molecule arrays, which we analyzed for both physical and biochemical insights within an int...Continue Reading

References

Sep 30, 1994·Science·A BensimonD Bensimon
Oct 15, 1995·Analytical Chemistry·A T Woolley, R A Mathies
Jul 1, 1997·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·T S AnantharamanD C Schwartz
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·J JingD C Schwartz
Oct 17, 1998·Science·M A BurnsD T Burke
Sep 29, 1999·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J SkiadasD C Schwartz
Feb 24, 2001·Nature·N T PernaF R Blattner
Jul 12, 2001·Annual Review of Biomedical Engineering·G M WhitesidesD E Ingber
May 30, 2002·Analytical Chemistry·J Cooper McDonaldGeorge M Whitesides
Jul 16, 2002·Annual Review of Biomedical Engineering·David J BeebeGlenn M Walker
Jul 27, 2002·Journal of Bacteriology·Wen DengRobert D Perry
Dec 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·R A WelchF R Blattner
Aug 9, 2003·Physical Review Letters·Richard M JendrejackJuan J de Pablo
Jun 1, 1962·Proceedings of the National Academy of Sciences of the United States of America·W E Streib, W N Lipscomb
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides

❮ Previous
Next ❯

Citations

Aug 3, 2005·Analytical and Bioanalytical Chemistry·Petra S Dittrich, Andreas Manz
Mar 15, 2008·Analytical and Bioanalytical Chemistry·Nicholas DouvilleShuichi Takayama
Jul 9, 2013·Biomedical Microdevices·Kaiping ChengGuodong Sui
Aug 8, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Teresa FazioEric C Greene
Sep 16, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Mari-Liis VisnapuuEric C Greene
Mar 18, 2009·Nano Letters·Yuval EbensteinShimon Weiss
May 16, 2009·Nature Protocols·Julia M SidorovaRaymond J Monnat
Jul 19, 2006·Nature Reviews. Genetics·Jian-Bing FanKevin L Gunderson
Mar 25, 2011·Lab on a Chip·Yoori KimDavid C Schwartz
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anton ValouevMichael S Waterman
Feb 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kyubong JoDavid C Schwartz
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brian TeagueDavid C Schwartz
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Martin TrebbinStephan Förster
Jun 15, 2007·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Haifeng LiLei M Li
Apr 7, 2006·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton ValouevMichael S Waterman
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Deepayan SarkarMichael A Newton
Sep 10, 2005·Applied and Environmental Microbiology·Susan ReslewicDavid C Schwartz
Nov 2, 2004·Journal of Bacteriology·Shiguo ZhouDavid C Schwartz
Feb 1, 2013·Journal of Clinical Microbiology·J Michael Miller
Jul 20, 2010·Annual Review of Analytical Chemistry·J William Efcavitch, John F Thompson
Aug 4, 2012·BMC Bioinformatics·Henry C LinMihai Pop
Jan 17, 2009·BMC Genomics·Chia-wei WuAdel M Talaat
Aug 19, 2007·BMC Genomics·Shiguo ZhouDavid C Schwartz
Aug 1, 2008·BMC Molecular Biology·Gene E AnanievDavid C Schwartz
May 27, 2009·PLoS Biology·Deanna M ChurchUNKNOWN Mouse Genome Sequencing Consortium
Nov 26, 2009·PLoS Genetics·Shiguo ZhouDavid C Schwartz
Aug 7, 2009·The Journal of Chemical Physics·Juan P Hernández-OrtizJuan J de Pablo
Jun 6, 2013·International Journal of Genomics·Aurélien J MazurieGregory A Buck
Aug 27, 2011·Biomicrofluidics·Shuang Fang LimRobert Riehn
Feb 3, 2012·Biomicrofluidics·Yanwei WangKevin D Dorfman
Jan 1, 2010·Journal of Computer Science and Technology·David C Schwartz, Michael S Waterman
Sep 15, 2015·Nucleic Acids Research·Dmitry Torchinsky, Yuval Ebenstein
Jun 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Aditya GuptaDavid C Schwartz
Aug 13, 2015·Nucleic Acids Research·Seonghyun LeeDavid C Schwartz
Dec 8, 2015·Bioinformatics·Lee M MendelowitzMihai Pop
Mar 24, 2010·Analytical Biochemistry·Ekaterina ProtozanovaRudolf Gilmanshin
Sep 15, 2009·Protein Expression and Purification·Penghua ZhangShuang-yong Xu
Apr 10, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Younghye KimBaek-Hui Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.