A microfluidics approach to the problem of creating separate solution environments accessible from macroscopic volumes

Analytical Chemistry
Jessica OlofssonOwe Orwar

Abstract

We report on a microfluidic device that generates separate solution environments in macroscopic volumes. Spatially distinct patterns are created by emitting fluids from 16 different sources (closely spaced microchannels) into a solution-filled macroscopic chamber. The fluid in neighboring microchannels couples viscously in the macroscopic container, generating one single interdigitated stream. Scanning nanoelectrode amperometry was used for characterizing the concentration landscape and the diffusion zones between solutions running in parallel at different coordinates in the stream. These experiments were complemented by finite element simulations of the Navier-Stokes and mass transport equations to describe the velocity distributions and the diffusion behavior. For in channel flow velocities of 50 mm.s(-1), patterns could persist on the order of millimeters to centimeters in the open volume. The most narrow diffusion zones with widths less than 10 microm (5-95% concentration change) were found some tens of micrometers out in the macroscopic container. We demonstrate that a 14-microm-diameter nearly spherical object (biological cell) attached to a micropipet can be moved from one solution environment to another by a lateral dis...Continue Reading

References

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Feb 10, 2006·Analytical Chemistry·Jon SinclairOwe Orwar

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Citations

Sep 14, 2010·Analytical and Bioanalytical Chemistry·Liaoran CaoMichael G Roper
Oct 24, 2013·Analytical and Bioanalytical Chemistry·Mark D TarnNicole Pamme
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jessica OlofssonOwe Orwar
Apr 5, 2011·Annual Review of Analytical Chemistry·Jason S Kuo, Daniel T Chiu
Nov 8, 2014·Biomicrofluidics·Dingsheng LiuDaniel T Chiu
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Benjamin P CasavantDavid J Beebe
Jun 2, 2009·Journal of Neuroscience Methods·Serguei S SidachMerouane Bencherif
Aug 21, 2007·Biochemical Pharmacology·John DunlopGeorg C Terstappen
Jun 8, 2007·Journal of Forensic Sciences·Suzanne C Bell, Rebecca D Hanes
Feb 10, 2010·IEEE Transactions on Nanobioscience·Akira YamadaKeiji Naruse
Oct 29, 2005·Drug Discovery Today·Johan PihlDaniel T Chiu
Feb 22, 2012·Journal of Neuroscience Methods·Nikolai FedorovMerouane Bencherif
Mar 4, 2014·Molecular Pharmacology·Adam P HillJamie I Vandenberg
Feb 7, 2009·Analytical Chemistry·Jessica OlofssonOwe Orwar
Nov 16, 2007·Analytical Chemistry·Helen BridleOwe Orwar
Dec 7, 2007·Analytical Chemistry·Maria MillingenOwe Orwar
Sep 6, 2013·Analytical Chemistry·Jessica OlofssonOwe Orwar
Feb 23, 2007·Analytical Chemistry·Mikael EvanderJohan Nilsson
Apr 13, 2006·Journal of the American Chemical Society·Jon SinclairOwe Orwar
Jun 15, 2006·Analytical Chemistry·Petra S DittrichAndreas Manz

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