PMID: 45785Dec 1, 1978

A micromethod for preparation of human macrophage cultures for the study of lymphocyte-macrophage interaction in immune interferon production and blastogenesis

Immunopharmacology
D GoldblattL B Epstein

Abstract

A new economical and reproducible micromethod for the preparation of human macrophage cultures in wells of a microtiter plate is described. The technique has been employed for the study of events which occur in the interaction of lymphocytes with macrophages in PHA-stimulated immune interferon production and blastogenesis. By comparison with the current Leighton tube macroculture systems, the microculture technique yielded a 7-fold increase in the number of macrophage cultures and a 5-fold increase in the number of T lymphocyte macrophage cultures from a given volume of blood. The replicability from sample to sample with regard to 3H-thymidine incorporation and amount of interferon produced is better in the microculture system than in the Leighton tube system. Such a microculture technique will thus provide a system whereby ready analysis of monocyte-macrophage function and interaction with lymphocytes in numerous disease states can now be realized.

References

Jul 1, 1981·Journal of Clinical Immunology·L B Epstein, S Gupta
Feb 1, 1981·Journal of Interferon Research·M Wiranowska-Stewart, W E Stewart
Mar 1, 1991·Cellular Immunology·A M Kesson, Y J Bryson
Mar 10, 2010·Neonatal Network : NN·Wanda G Bradshaw

Citations

Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·L B EpsteinT C Merigan

Related Concepts

T-Lymphocyte
Thymidine
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Human leukocyte interferon
Microplate
Interferon
Lymphocyte Activation
Interferons
Cell Adhesion
Macrophage

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