PMID: 9420473Jan 1, 1997Paper

A microsatellite-based MHC genotyping system for house mice (Mus domesticus)

Hereditas
S Meagher, W K Potts

Abstract

Major histocompatibility complex (MHC) genes are the most polymorphic loci known for vertebrates. Although this has been known for over two decades, the selective forces maintaining this genetic diversity are unclear. Efforts to study selection on these loci in nature have been hampered because no simple MHC typing systems are available. Here, we describe and evaluate a microsatellite-based MHC genotyping system for house mice (Mus domesticus). Thirty-five MHC-linked microsatellite loci were tested for amplification and scoring reliability, and 21 were deemed useful. These 21 loci were efficient at discriminating among nine serologically distinct MHC haplotypes, with 52% of microsatellite pairs providing 100% resolution. Since these microsatellite loci are scattered across the entire MHC region, they will be effective at detecting recombinant haplotypes. The number of alleles is higher for microsatellites inside the MHC than outside it, which presumably reflects genetic hitchhiking with MHC alleles under balancing selection. This microsatellite typing system now allows testing hypotheses about the nature of selection operating on MHC genes in natural populations of M. domesticus and other murid rodent species.

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Citations

Mar 1, 2010·Molecular Ecology Resources·W Babik
Jun 14, 2013·Ecology and Evolution·Anna K LindholmBarbara König
Mar 17, 2015·PLoS Genetics·Jacob A TennessenMichael S Blouin
Mar 18, 2015·International Journal of Molecular Sciences·Jiajun JiangXubo Wang
Oct 30, 2012·Molecular Biology and Evolution·Camille RouxXavier Vekemans
Sep 30, 2020·Biology of Reproduction·Ayumi HasegawaAtsuo Ogura
Aug 1, 1998·Trends in Ecology & Evolution·S V Edwards, P W Hedrick

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