PMID: 9443382Jan 27, 1998Paper

A microtechnique for the analysis of free and conjugated indole-3-acetic acid in milligram amounts of plant tissue using a benchtop gas chromatograph-mass spectrometer

Planta
D M RibnickyJ D Cohen

Abstract

A microtechnique was developed for the quantification of indole-3-acetic acid (IAA) in plant samples of one milligram fresh weight or less. The method permitted quantification of both free and conjugated IAA using a benchtop gas chromatograph-mass spectrometer. New methods for sample purification with high recovery at microscale levels, together with simple changes that result in enhanced sensitivity of the instrumentation, allowed for a significant reduction in the amount of plant material required for analysis. Single oat (Avena sativa L.) coleoptile tips could be studied with this method and were found to contain free and total IAA levels of 137 and 399 pg.mg-1 fresh weight, respectively. A single 5-d-old Arabidopsis thaliana (L.) Heynh. seedling was shown to contain 61 pg.mg-1 fresh weight free IAA and 7850 pg.mg-1 fresh weight of total IAA following basic hydrolysis. This microtechnique provides a way to accurately measure IAA levels in very small structures and individual seedlings, thus making it a valuable research tool for elucidating the role and distribution of auxin in relation to growth and development.

Citations

Dec 12, 2001·The Plant Journal : for Cell and Molecular Biology·K LjungG Sandberg
Dec 18, 2001·Plant Physiology·M Kowalczyk, G Sandberg
Jan 16, 2007·Plant Physiology·Yuko YamamotoTakashi Sazuka
Aug 3, 2011·Plant Physiology·Xing LiuGary Gardner
Aug 19, 2004·The Plant Journal : for Cell and Molecular Biology·Eric A SchmelzHans T Alborn
Nov 24, 1999·American Journal of Botany·A Ester SzteinT J Cooke
Oct 5, 2010·Nature Protocols·Lana S BarkawiJerry D Cohen
Sep 7, 2005·Photosynthesis Research·Pia Walch-LiuBrian G Forde
Mar 29, 2014·Planta·Danuše TarkowskáMiroslav Strnad
Nov 15, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jihong FuCunyu Yan

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