A microwell array device with integrated microfluidic components for enhanced single-cell analysis

Electrophoresis
Sara LindströmHelene Andersson-Svahn

Abstract

Increasing awareness of the importance of cell heterogeneity in many biological and medical contexts is prompting increasing interest in systems that allow single-cell analysis rather than conventional bulk analysis (which provides average values for variables of interest from large numbers of cells). Recently, we presented a microwell chip for long-term, high-throughput single-cell analysis. The chip has proved to be useful for purposes such as screening individual cancer and stem cells for protein/gene markers. However, liquids in the wells can only be added or changed by manually rinsing the chip, or parts of it. This procedure has several well-known drawbacks--including risks of cross-contamination, large dead volumes and laboriousness--but there have been few previous attempts to integrate liquid rinsing/switching channels in "ready-to-use" systems for single-cell analysis. Here we present a microwell system designed (using flow simulations) for single-cell analysis with integrated microfluidic components (microchannels, magnetically driven micropumps and reservoirs) for supplying the cell culture wells with reagents, or rinsing, thus facilitating controlled, directed liquid handling. It can be used totally independently, ...Continue Reading

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Citations

Apr 27, 2010·Analytical and Bioanalytical Chemistry·Donald Wlodkowic, Jonathan M Cooper
Jun 16, 2010·Analytical and Bioanalytical Chemistry·Iuliana OitaDebby Mangelings
Oct 23, 2010·Lab on a Chip·Sara Lindström, Helene Andersson-Svahn
May 19, 2012·PloS One·Matthijn C HesselmanMark W J van Passel
Oct 12, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kihoon JangTakehiko Kitamori
May 24, 2011·World Journal of Clinical Oncology·Donald Wlodkowic, Zbigniew Darzynkiewicz
Mar 8, 2012·Bioanalysis·Yong LuoNing Fang
Jan 1, 2012·Biomicrofluidics·Francesco PirainoAli Khademhosseini
Sep 22, 2015·International Journal of Molecular Sciences·Jie-Long HeShih-Kang Fan
Aug 15, 2015·International Journal of Molecular Sciences·Chansavath PhetsouphanhAnthony Dominic Kelleher
Nov 1, 2010·Expert Opinion on Drug Discovery·Dario Lombardi, Petra S Dittrich
May 11, 2010·Biochimica Et Biophysica Acta·Sara Lindström, Helene Andersson-Svahn
Sep 5, 2013·Advanced Drug Delivery Reviews·Kae SatoKiichi Sato
May 15, 2010·Expert Review of Molecular Diagnostics·Donald Wlodkowic, Jonathan M Cooper
Sep 26, 2020·Scientific Reports·Lauren RomitaDae Kun Hwang
Nov 28, 2012·Lab on a Chip·Nima JokilaaksoRashid Bashir

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