A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

Nature Structural & Molecular Biology
Lina VasiliauskaiteDónal O'Carroll

Abstract

In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI-piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI.

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Citations

Jul 29, 2020·Nature Communications·Theresa SchöppDónal O'Carroll
Sep 19, 2017·The Journal of Clinical Investigation·Gregory A WassermanMatthew R Jones
May 8, 2018·Nature Structural & Molecular Biology·Lina VasiliauskaitėDónal O'Carroll
Nov 18, 2018·Nature Reviews. Genetics·Deniz M OzataPhillip D Zamore
Nov 12, 2017·Nature Communications·Christina ErnstClaudia Kutter

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