A millifluidic study of cell-to-cell heterogeneity in growth-rate and cell-division capability in populations of isogenic cells of Chlamydomonas reinhardtii

PloS One
Shima P DamodaranFrancis-André Wollman

Abstract

To address possible cell-to-cell heterogeneity in growth dynamics of isogenic cell populations of Chlamydomonas reinhardtii, we developed a millifluidic drop-based device that not only allows the analysis of populations grown from single cells over periods of a week, but is also able to sort and collect drops of interest, containing viable and healthy cells, which can be used for further experimentation. In this study, we used isogenic algal cells that were first synchronized in mixotrophic growth conditions. We show that these synchronized cells, when placed in droplets and kept in mixotrophic growth conditions, exhibit mostly homogeneous growth statistics, but with two distinct subpopulations: a major population with a short doubling-time (fast-growers) and a significant subpopulation of slowly dividing cells (slow-growers). These observations suggest that algal cells from an isogenic population may be present in either of two states, a state of restricted division and a state of active division. When isogenic cells were allowed to propagate for about 1000 generations on solid agar plates, they displayed an increased heterogeneity in their growth dynamics. Although we could still identify the original populations of slow- and...Continue Reading

References

Aug 5, 1976·Nature·J L Spudich, D E Koshland
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M E PetracekJ Berman
May 31, 1990·Nature·C B HarleyC W Greider
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferJ P Aubert
Apr 1, 1980·The Journal of Cell Biology·J L Spudich, R Sager
Sep 1, 1995·European Journal of Endocrinology·H C HoeckP Laurberg
Jan 1, 1995·Annual Review of Genetics·J D Rochaix
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·H H McAdams, A Arkin
Oct 24, 1998·Biochimica Et Biophysica Acta·M HipplerJ D Rochaix
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai
Oct 9, 1999·Yeast·M G Barker, R M Walmsley
Mar 14, 2000·Current Opinion in Plant Biology·A R Grossman
Apr 12, 2001·Molecular Microbiology·B J HaijemaD Dubnau
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Elizabeth H Harris
Jun 29, 2002·Nature Biotechnology·Woodring E Wright, Jerry W Shay
Jun 20, 1959·Nature·R K MORTIMER, J R JOHNSTON
Dec 11, 2003·Eukaryotic Cell·Arthur R GrossmanZhaoduo Zhang
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Byron F Brehm-Stecher, Eric A Johnson
Feb 3, 2005·PLoS Biology·Eric J StewartFrançois Taddei
Feb 22, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Junmin PanWilliam J Snell
Jun 16, 2005·Genetics·Thomas PröscholdAnnette W Coleman
Sep 20, 2005·Nature·Alejandro Colman-LernerRoger Brent
Oct 26, 2005·Photosynthesis Research·Jean-David Rochaix
Feb 1, 1932·Journal of Bacteriology·C D Kelly, O Rahn
Mar 30, 2006·Journal of Bioscience and Bioengineering·Pauline SpolaoreArsène Isambert
Feb 24, 2007·Current Opinion in Biotechnology·Stephen P MayfieldJulia Marin-Navarro
Jul 24, 2007·Journal of Bacteriology·Tim J StrovasMary E Lidstrom
Oct 13, 2007·Science·Sabeeha S MerchantArthur R Grossman
Feb 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ann Richmond
Apr 17, 2008·Nature Reviews. Microbiology·Rudolf Amann, Bernhard M Fuchs
Jun 20, 2008·Current Opinion in Microbiology·Kamila CzechowskaJan Roelof van der Meer
Jul 12, 2008·Annual Review of Cell and Developmental Biology·K A SteinkrausB K Kennedy
Aug 19, 2008·Journal of Bacteriology·Azusa InoueJun-ichi Kato
Nov 6, 2008·Annual Review of Genetics·Stephan EberhardFrancis-André Wollman
Jan 20, 1932·The Journal of General Physiology·O Rahn
Oct 31, 2009·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Antoine E RouxLuis A Rokeach

❮ Previous
Next ❯

Citations

Apr 7, 2016·Biomicrofluidics·Rico IllingGianaurelio Cuniberti
Oct 21, 2016·Analytical and Bioanalytical Chemistry·Vojtěch LedvinaKarel Klepárník
Apr 5, 2017·Analytica Chimica Acta·Renny E FernandezNathan S Swami
May 24, 2016·Lab on a Chip·Tomasz S KaminskiPiotr Garstecki
May 18, 2016·Lab on a Chip·Olgierd CybulskiPiotr Garstecki
Jul 12, 2016·Lab on a Chip·Milad Abolhasani, Klavs F Jensen
Apr 15, 2016·PloS One·Denis CottinetJérôme Bibette
Jun 30, 2019·Micromachines·Lena GorgannezhadNam-Trung Nguyen
Jan 28, 2020·Small·Morteza JeyhaniScott S H Tsai
Mar 1, 2019·Biotechnology for Biofuels·Pierre BodénèsChun-Yen Wang
Apr 5, 2017·Annual Review of Analytical Chemistry·Oliver J DresslerAndrew J deMello
Sep 29, 2019·Lab on a Chip·M SolsonaM Odijk
Oct 15, 2020·MSystems·Robyn J WrightJan-Ulrich Kreft
Sep 14, 2018·Current Opinion in Plant Biology·James G Umen
Aug 13, 2021·Archives of Microbiology·Djihane Yushrina Damoo, Dion G Durnford
Dec 5, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Moien AlizadehgiashiEugenia Kumacheva

❮ Previous
Next ❯

Methods Mentioned

BETA
flow-cytometry

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Genetics & Epigenetics of Aging

Dozens of genes are implicated in lifespan, and epigenetic changes during aging affect cell function. This feed focuses on the genetics and epigenetics of aging.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Aging-Associated Metabolic Disorders

Age is associated with many metabolic disorders including cardiovascular diseases, type 2 diabetes, stroke and heart disease. The mediators in aging process have been suggested to play a part in the cellular processes responsible for these metabolic disorders. Here is the latest research on aging-associated metabolic disorders.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aging Genetics (Keystone)

This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.