PMID: 6540058May 15, 1984Paper

A miniature molecular-sieving column assay for cytoplasmic vitamin A-binding proteins

Analytical Biochemistry
J H MillerL E King

Abstract

Cytoplasmic vitamin A-binding proteins were measured by a method using centrifugation of gel-exclusion columns and compared to the sucrose gradient method. The gel-exclusion method analyzed 18 samples simultaneously on one table-top centrifuge, while the sucrose gradient method required use of three ultracentrifuges to process 18 samples simultaneously. Multiple 2-min low-speed centrifugations of test cytosol applied to miniature molecular-sieving columns was a faster (1/2 the working time for 18 samples), more convenient, and more accurate method for measuring cytoplasmic vitamin A-binding proteins than was the sucrose gradient method. Using the same cytosol and [3H]retinoid preparations, the rapid gel-exclusion method results were only slightly lower than those obtained by radioimmunoassay but 50% higher than the values obtained by the sucrose gradient assay. The methodology described may be useful not only for cytoplasmic vitamin A-binding proteins but also for other similar binding protein assays.

References

Jan 1, 1978·Vitamins and Hormones·F Chytil, D E Ong
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D E Ong, F Chytil
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M M BashorF Chytil
Jan 1, 1968·The Journal of Clinical Endocrinology and Metabolism·S G Korenman
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A J Wittwer, C Wagner

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Citations

May 12, 1988·Biochimica Et Biophysica Acta·A K Daly, C P Redfern
Oct 11, 2012·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Kalpana ParvathaneniAndrew T Tsin
Sep 1, 1985·The Journal of Investigative Dermatology·R E Gates, L E King
Jan 1, 1987·The Journal of Investigative Dermatology·R E GatesL E Allred

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