PMID: 9630718Jun 19, 1998Paper

A miniaturized monolayer trough with variable surface area in the square-millimeter range

Biochimica Et Biophysica Acta
C DuschlH Vogel

Abstract

A new simple concept for a miniaturized monolayer trough is described. The overall monolayer area in the expanded state is approximately 150 mm2 and can be reduced by a factor of 2. The surface area is a function of the shape of the meniscus formed by the subphase and is controlled by the amount of water in the monolayer trough. The controlled compression of monolayers to a desired area per molecule with simultaneous observation of the lateral distribution of fluorescently labeled molecules is shown. A biological reaction between a specific antibody and lipid anchored peptide demonstrates the feasibility of monolayer experiments, which require only very small quantities of substance (in the pmol range). This trough might also be a valuable tool for the 2D crystallization of proteins at lipid layers via specific binding sites such as metal chelators.

Citations

Aug 17, 1999·Current Opinion in Structural Biology·H Brockman

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