A minimal role for selectins in the recruitment of leukocytes into the inflamed liver microvasculature

The Journal of Clinical Investigation
J WongP Kubes

Abstract

A two-step paradigm for leukocyte recruitment has been established in a number of tissues including the mesentery, skin, and muscle, and necessitates an initial rolling step via the selectins before firm leukocyte adhesion via the integrins. In view of the many inflammatory diseases that involve the liver, we investigated the importance of rolling and the selectins in the hepatic microvasculature and compared the responses to that of the commonly used mesentery or cremaster microvasculature. We visualized the liver microvasculature using intravital microscopy and we determined that within the liver the majority of leukocytes adhere within the sinusoids (80%) in response to a chemotactic stimulus such as FMLP (20% in postsinusoidal venules) whereas leukocytes adhere exclusively within postcapillary venules in tissue like the mouse cremaster. In the sinusoids, the adhesive response to FMLP is not dependent upon selectins inasmuch as adhesion was not reduced in the sinusoidal vessels of P-selectin-deficient mice or E-selectin/P-selectin- deficient animals in the presence or absence of L-selectin antibody. No rolling or adhesion was detected in response to FMLP in the selectin-deficient cremaster microvasculature. Immunoneutralizat...Continue Reading

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Citations

Oct 27, 2004·Der Anaesthesist·C WunderO Eichelbrönner
Jul 14, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Braedon McDonald, Paul Kubes
Nov 5, 2010·Cell and Tissue Research·Paulina M KowalewskaAlison E Fox-Robichaud
Apr 23, 2011·Journal of Neural Transmission·Chris J Weston, David H Adams
Oct 7, 2010·International Journal of Clinical Oncology·Nobuyoshi Hiraoka
Jun 14, 2002·Alcohol·Geoffrey HaydonDavid H Adams
May 26, 1999·Infectious Disease Clinics of North America·C Parent, P Q Eichacker
Oct 22, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Christopher G. Kevil
Jun 28, 2003·Trends in Molecular Medicine·Klaus Ley
Apr 23, 2013·Cellular & Molecular Immunology·Fenglei Li, Zhigang Tian
Jan 23, 2013·Immunology and Cell Biology·Michael J Hickey, Clare L V Westhorpe
Sep 21, 2013·Nature Immunology·Craig N Jenne, Paul Kubes
Nov 9, 2011·Nature Medicine·Mia Phillipson, Paul Kubes
Dec 18, 2012·Nature Medicine·Sapna DeviMichael J Hickey
Feb 25, 2006·Nature Reviews. Immunology·David H Adams, Bertus Eksteen
Mar 1, 2002·Immunology and Cell Biology·Patricia F LalorDavid H Adams
Mar 1, 2002·Immunology and Cell Biology·Sun ParkI Nicholas Crispe
Apr 23, 2002·Journal of Gastroenterology and Hepatology·Sarah Goddard, David H Adams
May 5, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A Fox-Robichaud, P Kubes
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J A LawsonH Jaeschke
Apr 5, 2005·Toxicologic Pathology·George A Parker, Catherine A Picut
Dec 8, 1998·The Journal of Experimental Medicine·R C WoodmanP Kubes
May 11, 2002·Critical Care Medicine·John M Harlan, Robert K Winn
Feb 9, 2012·Cold Spring Harbor Perspectives in Medicine·William C Aird
Mar 1, 2000·Molecular Pathology : MP·P F Lalor, D H Adams
Dec 22, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Patricia F LalorDavid H Adams
Aug 31, 2012·Mediators of Inflammation·Evaggelia LiaskouYe H Oo
May 13, 2010·Digestive Diseases·Ye H OoDavid H Adams
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Apr 1, 2005·PLoS Biology·Frederic GeissmannDan R Littman
Sep 21, 2013·Annual Review of Pathology·Tanya N MayadasClifford A Lowell

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