A 'minimum dose' of lipopolysaccharide required for implantation failure: assessment of its effect on the maternal reproductive organs and interleukin-1alpha expression in the mouse

Reproduction : the Official Journal of the Society for the Study of Fertility
Kaushik DebYogesh K Jaiswal

Abstract

Genital tract infections caused by gram-negative bacteria induce abortion and are one of the most common complications of human pregnancy. This study was carried out to decipher the mechanism of gram-negative bacterial lipopolysaccharide (LPS)-induced pregnancy loss, using a mouse (Park strain) model. Since many of the biological effects of LPS are mediated by interleukin (IL)-1alpha, the role of IL-1alpha in LPS-induced pregnancy loss was studied. Pregnant female animals were injected intra-peritoneally (i.p.) with different doses (1 to 50 microg) of LPS from Salmonella minnesota Re-595, on day 0.5 of pregnancy. We found that 250 microg/kg body weight (i.e. 5 microg/female mouse) of LPS when given on day 0.5 of pregnancy was the 'minimum dose' (MD) required to completely inhibit the implantation of the blastocyst in the mouse. The effect of this dose on the pathophysiology of the various reproductive organs (i.e. uterus, ectoplacental cones, developing fetus, ovaries etc.) was assessed on day 14 of pregnancy. The effects of this dose on the level and pattern of expression of the proinflammatory cytokine IL-1alpha in the maternal uterine horns and preimplantation stage embryos were studied by RT-PCR. A single dose (100 ng/mouse...Continue Reading

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Citations

Jun 29, 2004·Archives of Gynecology and Obstetrics·Kaushik DebYogesh Kumar Jaiswal
Dec 24, 2011·Journal of Assisted Reproduction and Genetics·Varkha AgrawalYogesh Kumar Jaiswal
Feb 5, 2009·Reproductive Biomedicine Online·María L RibeiroAna M Franchi
Jul 20, 2012·Zygote : the Biology of Gametes and Early Embryos·Varkha AgrawalYogesh Kumar Jaiswal
Sep 2, 2006·Reproductive Biology and Endocrinology : RB&E·Simon MacKenzieJosep V Planas
May 9, 2007·Infectious Diseases in Obstetrics and Gynecology·Yogesh Kumar JaiswalKaushik Deb
Nov 12, 2014·Journal of Immunology Research·Xiaodan WangXiuhui Zhong
Aug 20, 2008·Fertility and Sterility·Yogesh Kumar JaiswalMadan Mohan Chaturvedi
Jan 16, 2009·American Journal of Reproductive Immunology : AJRI·Varkha AgrawalYogesh K Jaiswal
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Aug 11, 2011·Anatomy & Cell Biology·Jeong Mi MoonKwang Il Nam
Sep 26, 2017·Reproduction, Fertility, and Development·Katheryn L CernyPhillip J Bridges
Jan 25, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·K L BidneA F Keating
Jan 4, 2019·American Journal of Reproductive Immunology : AJRI·Sarah MoustafaShannon Whirledge
Nov 5, 2019·British Journal of Biomedical Science·W BahiaW Y Almawi
Mar 7, 2005·Reproductive Medicine and Biology·Kaushik DebYogesh Kumar Jaiswal
Sep 20, 2006·Human Reproduction·A F HirschfeldS E Turvey
Dec 10, 2020·Theriogenology·Fariborz MoghadamMohammad Hossein Nasr Esfahani
Jul 18, 2020·Molecular and Cellular Endocrinology·Upendra Kumar SoniRajesh Kumar Jha

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