A miRNA-101-3p/Bim axis as a determinant of serum deprivation-induced endothelial cell apoptosis

Cell Death & Disease
Ji-Hee KimYoung-Myeong Kim

Abstract

Serum deprivation or withdrawal induces apoptosis in endothelial cells, resulting in endothelial cell dysfunction that is associated with cardiovascular disease. However, there is still limited information on the role of miRNA in serum deprivation-induced apoptosis. Here we found that serum deprivation increased caspase-dependent apoptosis through miRNA-101-3p downregulation, without altering expression of its host gene RNA 3'-terminal phosphate cyclase-like 1, which was highly correlated with suppressed expression levels of Dicer and Argonaute 2 (Ago2), indicating that miR-101-3p is post-transcriptionally elevated in serum-deprived conditions. The decreased miR-101-3p caused elevated Bim expression by targeting its 3'-untranslated region (3'-UTR). This resulted in activation of the intrinsic pathway of apoptosis via interaction with Bcl-2, decreased mitochondrial membrane potential, cytochrome c release, mitochondrial reactive oxygen species (ROS) production, and caspase activation. These events were abrogated by miR-101-3p mimic and the proapoptotic Bim siRNA, which suggest a determinant role of the miR-101-3p/Bim axis in serum deprivation-induced apoptosis. The apoptosis induced by miR-101-3p-mediated Bim expression is media...Continue Reading

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Citations

Jan 29, 2020·Acta Biochimica Et Biophysica Sinica·Qiaoli ChenQianzhou Lv
Feb 28, 2018·Oncotarget·Huaqi WangGuoqiang Zhao
Apr 8, 2020·Experimental and Therapeutic Medicine·Huanxin ZhangJixian Zhao
May 16, 2020·Oxidative Medicine and Cellular Longevity·Can LuChen Chen
Apr 2, 2020·International Journal of Molecular Sciences·Simone MoertlMichael J Atkinson
Sep 20, 2020·Cell Death & Disease·Baochen BaiXian-Ming Chu
Jun 27, 2021·Molecular Biotechnology·Sooram Banesh, Vishal Trivedi

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Methods Mentioned

BETA
PCR
transfection
transfections
confocal microscopy

Software Mentioned

TargetScan

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