A misassembled transmembrane domain of a polytopic protein associates with signal peptide peptidase

The Biochemical Journal
Samuel G CrawshawStephen High

Abstract

The endoplasmic reticulum (ER) exerts a quality control over newly synthesized proteins and a variety of components have been implicated in the specific recognition of aberrant or misfolded polypeptides. We have exploited a site-specific cross-linking approach to search for novel ER components that may specifically recognize the misassembled transmembrane domains present in truncated polytopic proteins. We find that a single probe located in the transmembrane domain of a truncated opsin fragment is cross-linked to several ER proteins. These components are distinct from subunits of the Sec61 complex and represent a 'post-translocon' environment. In this study, we identify one of these post-translocon cross-linking partners as the signal peptide peptidase (SPP). We find that the interaction of truncated opsin chains with SPP is mediated by its second transmembrane domain, and propose that this interaction may contribute to the recognition of misassembled transmembrane domains during membrane protein quality control at the ER.

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Citations

Aug 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Akira SaitoYoshinori Akiyama
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michael B HarbutDoron C Greenbaum
Jun 4, 2011·The Journal of Biological Chemistry·Hiroyuki MiyashitaTakeshi Iwatsubo
Nov 5, 2008·The Journal of Biological Chemistry·Thalia RobakisPeter Scheiffele
Nov 10, 2009·Human Reproduction·Minako KoizumiYuji Taketani
Jan 27, 2009·Plant Physiology·Sungwon HanDanny J Schnell
May 23, 2012·Proteome Science·Stephen Hsueh-Jeng LuPeter St George-Hyslop
Jun 25, 2014·The Journal of Cell Biology·Jessica M BonamePaul J Lehner
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Oct 9, 2013·Biochimica Et Biophysica Acta·Matthias VossRegina Fluhrer
Jul 16, 2014·Biochemical and Biophysical Research Communications·Michael BaldwinAthar H Chishti
May 18, 2006·The Journal of Biological Chemistry·Steeve BoulantJohn McLauchlan
Jul 20, 2006·The Journal of Biological Chemistry·Christelle Vauloup-FellousArielle R Rosenberg
Apr 22, 2008·The Journal of Biological Chemistry·Paul Targett-AdamsJohn McLauchlan
Apr 25, 2007·Molecular Membrane Biology·Samuel G CrawshawStephen High
Apr 13, 2019·Glycobiology·Sarah O'KeefeStephen High
May 7, 2009·Journal of Cell Science·Benedict C S Cross, Stephen High
Aug 31, 2006·Journal of Cell Science·Andrew R J YoungSharon A Tooze

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