A misexpression screen reveals effects of bag-of-marbles and TGF beta class signaling on the Drosophila male germ-line stem cell lineage

Genetics
Cordula SchulzMargaret T Fuller

Abstract

Male gametes are produced throughout reproductive life by a classic stem cell mechanism. However, little is known about the molecular mechanisms for lineage production that maintain male germ-line stem cell (GSC) populations, regulate mitotic amplification divisions, and ensure germ cell differentiation. Here we utilize the Drosophila system to identify genes that cause defects in the male GSC lineage when forcibly expressed. We conducted a gain-of-function screen using a collection of 2050 EP lines and found 55 EP lines that caused defects at early stages of spermatogenesis upon forced expression either in germ cells or in surrounding somatic support cells. Most strikingly, our analysis of forced expression indicated that repression of bag-of-marbles (bam) expression in male GSC is important for male GSC survival, while activity of the TGF beta signal transduction pathway may play a permissive role in maintenance of GSCs in Drosophila testes. In addition, forced activation of the TGF beta signal transduction pathway in germ cells inhibits the transition from the spermatogonial mitotic amplification program to spermatocyte differentiation.

References

Nov 1, 1979·Journal of Ultrastructure Research·R W HardyM Garavito
Dec 1, 1990·Genes & Development·D M McKearin, A C Spradling
Sep 1, 1993·Mechanisms of Development·M Klingler, J P Gergen
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·P Rørth
Mar 21, 1998·Current Biology : CB·M Van DorenR Lehmann
Feb 26, 2000·Science·F M Watt, B L Hogan
Nov 2, 2001·Nature·A SpradlingT Kai
Jan 10, 2003·Nucleic Acids Research·UNKNOWN FlyBase Consortium
Jun 5, 2003·Developmental Biology·James J FabrizioStephen DiNardo
Sep 13, 2003·Science·Yukiko M YamashitaMargaret T Fuller

❮ Previous
Next ❯

Citations

Jul 11, 2008·Molecular Genetics and Genomics : MGG·Claudia LalancetteStephen A Krawetz
Jul 29, 2005·Cell and Tissue Research·Kate Lakoski Loveland, Gary Hime
Aug 12, 2005·Cell and Tissue Research·Catherine M BrowneKate L Loveland
Apr 20, 2013·Trends in Cell Biology·Rachel R Stine, Erika L Matunis
Oct 17, 2008·Nature·Jun ChengYukiko M Yamashita
Jul 14, 2010·Nature Cell Biology·Judith L Leatherman, Stephen Dinardo
Aug 4, 2011·Nature Communications·Marcus MichelChristian Bökel
Dec 21, 2006·Cell Research·Feng LiYibin Kang
Apr 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Thomas FlattMarc Tatar
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Megan L InscoMargaret T Fuller
Oct 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jaclyn G Y Lim, Margaret T Fuller
Nov 4, 2008·Briefings in Functional Genomics & Proteomics·Li-En JaoShawn M Burgess
Dec 21, 2006·Molecular Biology and Evolution·Rasika KalameghamBrian Oliver
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Yukiko M YamashitaAlan J Hunt
Jul 28, 2011·Development, Growth & Differentiation·Ziguang LiuShin Sugiyama
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Linheng Li, Ting Xie
Jun 23, 2011·Development·Margaret de Cuevas, Erika L Matunis
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yun LiJean Z Maines
Dec 3, 2015·Scientific Reports·Yalan Xing, Willis X Li
Feb 11, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ting XieMarco D Wong
Dec 18, 2008·The Journal of Pathology·M R WalkerT S Stappenbeck
Feb 26, 2009·Genesis : the Journal of Genetics and Development·Diana L van de HoefGabrielle L Boulianne
Jun 19, 2007·International Journal of Andrology·G R HimeH E Abud
Jan 25, 2007·Genesis : the Journal of Genetics and Development·Simon ReeveKevin G Moffat
Jun 20, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Leonard L Dobens, Samuel Bouyain
Jul 8, 2011·Genesis : the Journal of Genetics and Development·Jennifer C Jemc
Apr 11, 2012·Biotechnology Journal·Fani Papagiannouli, Ingrid Lohmann
Feb 16, 2016·Biochimica Et Biophysica Acta·Alexei A KotovLudmila V Olenina

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.