A misexpression study examining dorsal thorax formation in Drosophila melanogaster.

Genetics
María Teresa Peña-RangelJuan Rafael Riesgo-Escovar

Abstract

We studied thorax formation in Drosophila melanogaster using a misexpression screen with EP lines and thoracic Gal4 drivers that provide a genetically sensitized background. We identified 191 interacting lines showing alterations of thoracic bristles (number and/or location), thorax and scutellum malformations, lethality, or suppression of the thoracic phenotype used in the screen. We analyzed these lines and showed that known genes with different functional roles (selector, prepattern, proneural, cell cycle regulation, lineage restriction, signaling pathways, transcriptional control, and chromatin organization) are among the modifier lines. A few lines have previously been identified in thorax formation, but others, such as chromatin-remodeling complex genes, are novel. However, most of the interacting loci are uncharacterized, providing a wealth of new genetic data. We also describe one such novel line, poco pelo (ppo), where both misexpression and loss-of-function phenotypes are similar: loss of bristles and scutellum malformation.

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Citations

Oct 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Min LiYi-Hsin Liu
Jul 12, 2005·Vaccine·Consuelo AlmazánJosé de la Fuente
Dec 19, 2007·Nature Immunology·Bruce Beutler, Eva Marie Y Moresco
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Apr 17, 2012·The International Journal of Biochemistry & Cell Biology·Geneviève Morrow, Robert M Tanguay

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