PMID: 108517Mar 9, 1979

A missense mutation in the gene coding for ribosomal protein S17 (rpsQ) leading to ribosomal assembly defectivity in Escherichia coli

Molecular & General Genetics : MGG
A HerzogA Bollen

Abstract

The conditionally lethal mutation, 2861 mis, has been mapped inside the ribosomal protein gene cluster at 72 minutes on the Escherichia coli chromosome and was found to cotransduce at 97% with rpsE (S5). The 2861 mis mutation leads to thermosensitivity and impaired assembly in vivo of 30S ribosomal particles at 42 degrees C. The strain carrying the mutation has an altered S 17 ribosomal protein; the mutational alteration involves a replacement of serine by phenylalanine in protein S 17. Spontaneous reversion to temperature independence can restore the normal assembly in vivo of 30 S ribosomal subunits at 42 degrees C and the normal chromatographical behaviour of the S 17 ribosomal protein in vitro. We conclude therefore that the 2861 mis mutation affects the structural gene for protein S 17 (rpsQ).

References

Sep 1, 1975·Analytical Biochemistry·E Mendez, C Y Lai
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Nov 29, 1977·Molecular & General Genetics : MGG·G DelcuveA Bollen
Aug 10, 1976·Molecular & General Genetics : MGG·E DubnauI Smith
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Citations

Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·B A Maguire
Apr 18, 2015·BMC Molecular Biology·Rainer NikolayElke Deuerling
Jul 1, 2009·Journal of Molecular Biology·Christopher J Woolstenhulme, W E Hill
Jul 21, 2009·Journal of Molecular Biology·Priya Ramaswamy, Sarah A Woodson
May 31, 2016·Antibiotics·Rainer NikolayK H Nierhaus
Nov 13, 2004·FEMS Microbiology Reviews·Stefan SchwarzAxel Cloeckaert
Jan 28, 2003·Biopolymers·G M Culver
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·V RamakrishnanS W White

Related Concepts

Bacterial Proteins
Lactrase
Genome Mapping
Chromosomes, Bacterial
Alkalescens-Dispar Group
Cistron
Endorphenyl
Ribosomal Proteins
Serine
Transduction, Genetic

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