A mitochondrial-like targeting signal on the hydrogenosomal malic enzyme from the anaerobic fungus Neocallimastix frontalis: support for the hypothesis that hydrogenosomes are modified mitochondria

Molecular Microbiology
M van der GiezenR A Prins

Abstract

The hydrogenosomal malic enzyme (ME) was purified from the anaerobic fungus Neocallimastix frontalis. Using reverse genetics, the corresponding cDNA was isolated and characterized. The deduced amino acid sequence of the ME showed high similarity to ME from metazoa, plants and protists. Putative functional domains for malate and NAD+/NADP+ binding were identified. Phylogenetic analysis of the deduced amino acid sequence of the new ME suggests that it is homologous to reference bacterial and eukaryotic ME. Most interestingly, the cDNA codes for a protein which contains a 27-amino-acid N-terminus which is not present on the purified mature protein. This presequence shares features with known mitochondrial targeting signals, including an enrichment in Ala, Leu, Ser, and Arg, and the presence of an Arg at position-2 relative to amino acid 1 of the mature protein. This is the first report of a mitochondrial-like targeting signal on a hydrogenosomal enzyme from an anaerobic fungus and provides support for the hypothesis that hydrogenosomes in Neocallimastix frontalis might be modified mitochondria.

Citations

Nov 1, 1997·Trends in Ecology & Evolution·T M EmbleyR P Hirt
Mar 14, 2002·Gene·Frank G J VonckenJohannes H P Hackstein
Feb 3, 1998·Trends in Microbiology·H J Flint
Nov 5, 1999·Trends in Microbiology·J H HacksteinF G Voncken
Sep 6, 2000·Current Opinion in Microbiology·S D Dyall, P J Johnson
Feb 13, 1999·Molecular Microbiology·A AkhmanovaJ H Hackstein
Jun 1, 1998·Nutrition Research Reviews·G L Gordon, M W Phillips
Nov 9, 2000·Molecular Biology and Evolution·D S HornerT M Embley
Mar 21, 2000·Molecular Biology and Evolution·S Garcia-VallvéJ Palau
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T Martin EmbleyPeter Foster
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Siv G E AnderssonCharles G Kurland
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·C G Kurland, S G Andersson
Dec 1, 2010·IMA Fungus·Gareth W GriffithGordon Beakes
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J J Van HellemondA G Tielens
Jan 21, 2010·International Journal for Parasitology·Aloysius G M TielensWilliam Martin
Dec 1, 1998·Protist·E PlümperP J Johnson
Jan 26, 2007·International Journal for Parasitology·Lena Burri, Patrick J Keeling
Jun 17, 2009·The Journal of Eukaryotic Microbiology·Mark van der Giezen
May 3, 2014·Biotechnology and Bioengineering·Charles H HaitjemaMichelle A O'Malley
Dec 15, 2005·Zoology : Analysis of Complex Systems, ZACS·J H HacksteinM Veenhuis
Sep 2, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Courtney W StairsAndrew J Roger
Dec 1, 2004·Annual Review of Genetics·Michael W GrayGertraud Burger
Nov 21, 2007·The Journal of Biological Chemistry·Koen W A van GrinsvenKatrin Henze
Sep 6, 2020·Bulletin of Mathematical Biology·John R JungckLouis J Gross
Jul 2, 1999·Antimicrobial Agents and Chemotherapy·J Samuelson
May 1, 1997·Microbiology·Giancarlo A BiaginiDavid Lloyd
Feb 7, 1997·Science·J D Palmer

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