A mitochondrial nuclease is modified in Drosophila mutants (mus308) that are hypersensitive to DNA crosslinking agents

Molecular & General Genetics : MGG
Kengo SakaguchiJ B Boyd

Abstract

The mus308 mutants of Drosophila have previously been demonstrated to be defective in an enzyme that is designated Nuclease 3 (Boyd et al. 1990b). In this study that enzyme is shown to be present in mitochondria of both wild-type flies and embryos. Since the mus308 mutants are hypersensitive to DNA crosslinking agents. Nuclease 3 is potentially required for resistance of the mitochondrial genome to such agents. In support of this hypothesis, electron microscopic studies of mus308 mutant flies that had been exposed to nitrogen mustard revealed an increased frequency of mitochondrial abnormalities. Further investigation of the defect at the enzymological level revealed that the mutants possess a new nuclease activity that is apparently a modified form of the wild-type protein. In the earlier study, enzyme extracts from mus308 mutants were found to lack an enzyme with a pI of approximately 6.2. More precisely defined assay conditions in this study revealed the appearance of a new nuclease activity with a higher pI in extracts from mutants. This observation, together with the finding that only the normal enzyme form is present in heterozygous individuals, supports the hypothesis that the mus308 locus is not the structural gene for ...Continue Reading

References

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Citations

Dec 1, 1994·Mutation Research·L F Povirk, D E Shuker
Jan 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G D Baldridge, A M Fallon
Jul 1, 1991·Mutation Research·K SakaguchiJ B Boyd
Jun 8, 2002·Journal of Molecular Biology·Giovanni MagaUlrich Hübscher
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Youssoufian
Oct 28, 2006·Radiation Research·Joann B SweasyKristin A Eckert
Feb 1, 1993·Mutation Research·E A Leonhardt, J B Boyd
Apr 16, 1998·Mutation Research·P G de Buendía
May 12, 2004·Protein Expression and Purification·Masahiko OshigeKengo Sakaguchi
Jan 22, 2009·The FEBS Journal·Kengo SakaguchiKazuki Iwabata

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