A mixed-mode resin with tryptamine ligand for human serum albumin separation

Journal of Chromatography. a
Qi-Ci WuShan-Jing Yao

Abstract

Mixed-mode chromatography (MMC) is a new technology that uses specially-designed ligands to improve the adsorption selectivity with multimodal protein-ligand interactions for protein separation. A new MMC resin TA-B-6FF with tryptamine as the functional ligand was prepared and used for human serum albumin (HSA) separation. Adsorption equilibria of plasma-derived HSA (pHSA) were investigated and compared with a commercial tryptophan-based resin (MX-Trp-650m), and the influence of pH and salt addition was studied. The results showed that weak acidic conditions (pH 5.0-7.0) were favorable for HSA adsorption. The maximum adsorption capacity of TA-B-6FF was 141.33mg/g at pH 5.0, which was two times higher than that of MX-Trp-650m. TA-B-6FF also showed better salt-tolerance than MX-Trp-650m. Moreover, TA-B-6FF was used to separate recombinant HSA (rHSA) from Pichia pastoris culture broth. The results indicated that rHSA could be directly captured by TA-B-6FF without dilution or pH adjustment. High purity (87.75%) of rHSA monomer could be obtained with a recovery of 98.53% through two-step elution process. Total content of rHSA monomer and degraded fragment was 99.75%, the removal of host cell proteins reached about 90%. The results d...Continue Reading

References

May 6, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·W E Müller, U Wollert
Jul 16, 1992·Nature·X M He, D C Carter
Jul 20, 1990·Science·D C Carter, X M He
Jun 9, 1989·Science·D C CarterT Y Miller
Jan 1, 1994·Advances in Protein Chemistry·D C Carter, J X Ho
May 5, 1999·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·K KobayashiK Yokoyama
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Ulrich Kragh-HansenMasaki Otagiri
Dec 27, 2005·Best Practice & Research. Clinical Haematology·Sheila MacLennan, John A J Barbara
Mar 23, 2006·World Journal of Gastroenterology : WJG·Roberto-Giulio RomanelliGiacomo Laffi
Oct 5, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dong GaoShan-Jing Yao
Oct 11, 2007·Drugs of Today·Victor T G Chuang, Masaki Otagiri
Mar 31, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun RenJian Xie
May 5, 2009·Journal of Biotechnology·Guofeng ZhaoYan Sun
May 7, 2013·Biochimica Et Biophysica Acta·Zhen ChenDaichang Yang
Jul 28, 2013·Journal of Chromatography. a·Lin-Ling Yu, Yan Sun

❮ Previous
Next ❯

Citations

Nov 15, 2018·Journal of Separation Science·David SýkoraVladimír Král
Sep 8, 2020·Journal of Separation Science·Cheng-Tong GeDong-Qiang Lin
Apr 24, 2019·Journal of Chromatography. a·Jiali GuLi Xiao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.