Abstract
Chemotaxis may be studied in two main ways: 1) counting cells passing through an insert (e.g., using Boyden chambers), and 2) directly observing cell cultures (e.g., using Dunn chambers), both in response to stationary concentration gradients. This article promotes the use of Dunn chambers and in vitro cell-tracking, achieved by video microscopy coupled with automatic image analysis software, in order to extract quantitative and qualitative measurements characterizing the response of cells to a diffusible chemical agent. Previously, we set up a videomicroscopy system coupled with image analysis software that was able to compute cell trajectories from in vitro cell cultures. In the present study, we are introducing a new software increasing the application field of this system to chemotaxis studies. This software is based on an adapted version of the active contour methodology, enabling each cell to be efficiently tracked for hours and resulting in detailed descriptions of individual cell trajectories. The major advantages of this method come from an improved robustness with respect to variability in cell morphologies between different cell lines and dynamical changes in cell shape during cell migration. Moreover, the software i...Continue Reading
References
Mar 1, 1974·The Journal of Cell Biology·M A GimbroneJ Folkman
Mar 1, 1997·British Journal of Haematology·D ZichaA W Segal
Mar 17, 1997·Biochemical and Biophysical Research Communications·C De HauwerP Van Ham
Jun 12, 1998·The Journal of Cell Biology·W E AllenG E Jones
Jul 23, 1998·British Journal of Haematology·D ZichaA J Thrasher
Nov 25, 1998·Journal of Neurobiology·C De HauwerR Kiss
May 20, 1999·Cytometry·C De HauwerC Decaesteker
Nov 25, 2000·Journal of Pharmacological and Toxicological Methods·S FarinelleR Kiss
Apr 17, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·N NagyC Decaestecker
Feb 13, 2002·Gut·N NagyH-J Gabius
Feb 19, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Carine DelbrouckRobert Kiss
Jul 12, 2002·Cell Motility and the Cytoskeleton·Dirk DormannTill Bretschneider
Citations
Aug 22, 2009·Clinical Oral Investigations·Dan Dominik BrüllmannBernd d'Hoedt
Oct 16, 2010·Clinical Oral Investigations·Dan Dominik BrüllmannBernd d'Hoedt
Mar 12, 2010·Tissue Engineering. Part C, Methods·Hans PolzerMatthias Schieker
Jul 18, 2009·Algorithms for Molecular Biology : AMB·Axel MosigSu-Shing Chen
Jan 10, 2014·PloS One·Rajib ChakravortyPhilip D Hodgkin
Aug 8, 2009·Seminars in Cell & Developmental Biology·Erik MeijeringWiggert A van Cappellen
Sep 19, 2015·Scientific Reports·Smruti M PhadnisRenee A Reijo Pera
Feb 4, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Omar I ButtNicanor I Moldovan
Aug 12, 2009·Small·Elisabet Fernandez-RosasJosé Antonio Plaza
Jul 1, 2009·International Journal of Experimental Pathology·Carolyn A StatonNicola J Brown
Feb 7, 2009·Journal of Microscopy·J DegermanT Gustavsson
Nov 18, 2006·Cell Proliferation·A TárnokG Brockhoff
Dec 9, 2010·Genesis : the Journal of Genetics and Development·Khaled Khairy, Philipp J Keller
Mar 5, 2014·IEEE Journal of Biomedical and Health Informatics·Ketheesan ThirusittampalamPaul F Whelan
Jul 24, 2014·IEEE Journal of Biomedical and Health Informatics·Wojciech TarnawskiAldo Ferrari
Sep 30, 2005·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Alexandre DufourChristophe Zimmer
Nov 4, 2006·Microvascular Research·K C ChawS Swaminathan
Jul 12, 2005·Toxicology and Applied Pharmacology·Caroline HayotChristine Decaestecker
Feb 14, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ching-Fen JiangMing-Hong Tsai
Nov 18, 2016·Scientific Reports·Joe ChalfounMary Brady
Aug 5, 2006·Medicinal Research Reviews·Christine DecaesteckerRobert Kiss
Jun 2, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Shan JiangStephen T C Wong
Oct 17, 2015·BMC Bioinformatics·Peter BajcsyMary Brady
Jun 2, 2020·Journal of Motor Behavior·Michael J Hiley, Maurice R Yeadon
Nov 6, 2012·Journal of Biophotonics·Susanne SchäferDieter Dirksen
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Florence LefrancRobert Kiss