A model cell line to study regulation of stearoyl-CoA desaturase gene 1 expression by insulin and polyunsaturated fatty acids

Biochemical and Biophysical Research Communications
J M NtambiT Takova

Abstract

Insulin and polyunsaturated fatty acids (PUFAs) regulate the expression of SCD1 gene in mouse liver. Accordingly, we examined the insulin and PUFA regulation of SCD1 gene expression in H2.35 cells. The levels of SCD1 mRNA in H2.35 cells increased at the restrictive temperature of 39 degrees C, when the glucose-containing medium was supplemented with insulin. The insulin-stimulated expression of SCD1 mRNA was significantly blunted when the induction medium was supplemented with linolenic acid (18:2n-3) and arachidonic acid (20:4n-6). Stearic acid (18:0n-9) and oleic acid (18:1n-9) were without dramatic effects. The effect of insulin and PUFAs on a transfected SCD1 fusion gene (SCD1.CAT4.3) was also examined in H2.35 cells. Whereas insulin stimulated SCD1.CAT4.3 expression, arachidonic acid significantly decreased SCD1.CAT4.3 activity. These studies suggest that insulin and PUFAs regulate SCD1 gene transcription via regulatory DNA sequences flanking the 5' end of the gene.

Citations

Jan 6, 2012·Pflügers Archiv : European journal of physiology·Pallavi CheguruRodney A Hill
Jul 19, 2001·Journal of the National Cancer Institute·V PalaF Berrino
Jun 11, 2004·Annual Review of Nutrition·Manabu T Nakamura, Takayuki Y Nara
Feb 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Thomas SaetherTrine B Haugen
Sep 25, 2012·Progress in Lipid Research·Leanne Hodson, Barbara A Fielding
Aug 18, 2010·Biochimie·Daniel Mauvoisin, Catherine Mounier
Apr 6, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shu-Ling HsiehTian-Jye Hsieh
Sep 24, 2015·Journal of Agricultural and Food Chemistry·Stephanie K GatrellXin Gen Lei
Dec 1, 2010·Journal of Animal Physiology and Animal Nutrition·I J KarlengenO Taugbøl
Oct 26, 2001·The Journal of Biological Chemistry·William SamuelBarbara Wiggert
Jul 27, 2000·The Journal of Biological Chemistry·A H HastyN Yamada
Nov 9, 2005·The Journal of Biological Chemistry·Weng Chi ManJames M Ntambi
Dec 3, 1999·Biochemical and Biophysical Research Communications·Y C Kim, J M Ntambi
May 23, 1998·Biochimica Et Biophysica Acta·R J WardM C Barber
Nov 26, 1998·Progress in Lipid Research·D R TocherP A Hodgson
Oct 6, 1999·Prostaglandins, Leukotrienes, and Essential Fatty Acids·C L LoizouC N Hales
Dec 31, 2002·Biochemical and Biophysical Research Communications·F Enrique GomezBrian G Fox
Jan 23, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Makoto Miyazaki, James M Ntambi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.