PMID: 12788506Jun 6, 2003Paper

A model experimental system for monitoring changes in sensory neuron phenotype evoked by tooth injury

Journal of Neuroscience Methods
Yan PanJ P Naftel

Abstract

The dental pulp is a favorable model for studies of interactions between nociceptive sensory neurons and their peripheral target tissues. In the present study, we retrogradely labeled pulpal afferent neurons with an improved method that permits monitoring of changes in neuronal phenotype in response to controlled tooth injuries. The capacity of retrograde neuronal tracers to diffuse through dentinal tubules was exploited, thereby avoiding the severe injury to the pulp associated with previous tracer application methods. The strategy was to apply the durable fluorescent tracer, Fluoro-gold (FG), to exposed dentin in the floor of shallow cavities in molars, in order to pre-label pulpal neurons in trigeminal ganglia of young adult Sprague-Dawley rats. A high percentage of pupal afferent neurons were retrogradely labeled by application of FG to exposed dentin and the FG fluorescent signal persisted in most labeled neurons for at least 8 weeks. Following tracer application to dentin, the pulp tissue appeared normal histologically, with the exception that a layer of reactive dentin was deposited at the pulp-dentin border beneath the shallow cavities. Assessment of expression of calcitonin gene-related peptide (CGRP) and brain derived...Continue Reading

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Citations

Mar 17, 2011·Journal of Dental Research·J L GibbsA I Basbaum
Jun 7, 2012·Journal of Dental Research·M-K ChungX Dong
Aug 23, 2008·Journal of Dental Research·T O HermanstyneM S Gold
Mar 3, 2005·Archives of Oral Biology·Eric H Chudler, Margaret R Byers
Apr 8, 2017·Journal of Dental Research·J WonS B Oh
Oct 26, 2006·Journal of Dental Research·H E GoodisK M Hargreaves
Jun 14, 2017·Surgical Neurology International·Syed M AndrabiAbdul R Bhat
Apr 9, 2019·Journal of Dental Research·C R DonnellyB A Pierchala
Nov 14, 2019·Journal of Dental Research·P R LeeS B Oh

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