PMID: 3377502Apr 1, 1988

A model for analyzing growth kinetics of a slowly growing Mycobacterium sp

Applied and Environmental Microbiology
R S LambrechtMichael T Collins


This report describes a simple method for quantifying viable mycobacteria and for determining generation time. We used statistical models and computer analysis of growth curves generated for the slowly growing mycobacterium Mycobacterium paratuberculosis under controlled conditions to derive a mathematical formula relating the dependent variable, growth, to the independent variables, log10 number of organisms in the inoculum (inoculum size) and incubation time. Growth was measured by a radiometric method which detects 14CO2 release during metabolism of a 14C-labeled substrate. The radiometric method allowed for early detection of growth and detected as few as three viable bacteria. The coefficient of variation between culture vials inoculated with the same number of M. paratuberculosis was 0.083. Radiometric measurements were highly correlated to spectrophotometric and plate count methods for measuring growth (r = 0.962 and 0.992, respectively). The proportion of the total variability explained by the model in a goodness of fit test was 0.9994. Application of the model to broth cultures provided accurate estimates of the number of M. paratuberculosis (standard error = 0.21, log10 scale) and the growth rate (coefficient of varia...Continue Reading


Jul 25, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D S MillerA Hope
Nov 7, 2003·Applied and Environmental Microbiology·N Sung, Michael T Collins
Jun 9, 2005·Clinical and Diagnostic Laboratory Immunology·Michael T CollinsR H Whitlock
Feb 16, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·N Beth HarrisJanet B Payeur
Apr 8, 2006·Letters in Applied Microbiology·M T Rowe, I R Grant
Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Raymond W SweeneySusan C McAdams
May 22, 2009·The Journal of Antimicrobial Chemotherapy·Manju Y KrishnanMichael T Collins
Mar 8, 2011·Journal of Food Protection·C O GillW J Meadus
Oct 1, 1990·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·R M van BoxtelMichael T Collins
Jun 6, 2003·Applied and Environmental Microbiology·Leslie A ReddacliffRichard J Whittington
Oct 19, 2004·Annual Review of Microbiology·Ofelia ChaconRaúl G Barletta
Dec 12, 2007·Antimicrobial Agents and Chemotherapy·Sung Jae Shin, Michael T Collins
Sep 11, 2010·Expert Review of Clinical Immunology·Alka A Desai, Daniel J B Marks
Apr 10, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sung G KimDonald A Lein
Jul 3, 2016·Applied and Environmental Microbiology·Hannah B PooleyKarren M Plain
Jan 15, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·A LewinB Appel
Nov 2, 2014·Frontiers in Cellular and Infection Microbiology·Govardhan RathnaiahRaúl G Barletta
Oct 18, 2011·Applied and Environmental Microbiology·Natalia ElguezabalRamón A Juste
Jun 16, 2018·F1000Research·Ashwani KesarwaniPramod Upadhyay

Related Concepts

Carbon Radioisotopes
Genus Mycobacterium
Palmitic Acids
Sodium Palmitate

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.