PMID: 3377502Apr 1, 1988

A model for analyzing growth kinetics of a slowly growing Mycobacterium sp

Applied and Environmental Microbiology
R S LambrechtMichael T Collins

Abstract

This report describes a simple method for quantifying viable mycobacteria and for determining generation time. We used statistical models and computer analysis of growth curves generated for the slowly growing mycobacterium Mycobacterium paratuberculosis under controlled conditions to derive a mathematical formula relating the dependent variable, growth, to the independent variables, log10 number of organisms in the inoculum (inoculum size) and incubation time. Growth was measured by a radiometric method which detects 14CO2 release during metabolism of a 14C-labeled substrate. The radiometric method allowed for early detection of growth and detected as few as three viable bacteria. The coefficient of variation between culture vials inoculated with the same number of M. paratuberculosis was 0.083. Radiometric measurements were highly correlated to spectrophotometric and plate count methods for measuring growth (r = 0.962 and 0.992, respectively). The proportion of the total variability explained by the model in a goodness of fit test was 0.9994. Application of the model to broth cultures provided accurate estimates of the number of M. paratuberculosis (standard error = 0.21, log10 scale) and the growth rate (coefficient of varia...Continue Reading

Citations

Jul 25, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D S MillerA Hope
Nov 7, 2003·Applied and Environmental Microbiology·N Sung, Michael T Collins
Jun 9, 2005·Clinical and Diagnostic Laboratory Immunology·Michael T CollinsR H Whitlock
Feb 16, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·N Beth HarrisJanet B Payeur
Apr 8, 2006·Letters in Applied Microbiology·M T Rowe, I R Grant
Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Raymond W SweeneySusan C McAdams
May 22, 2009·The Journal of Antimicrobial Chemotherapy·Manju Y KrishnanMichael T Collins
Mar 8, 2011·Journal of Food Protection·C O GillW J Meadus
Oct 1, 1990·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·R M van BoxtelMichael T Collins
Jun 6, 2003·Applied and Environmental Microbiology·Leslie A ReddacliffRichard J Whittington
Oct 19, 2004·Annual Review of Microbiology·Ofelia ChaconRaúl G Barletta
Dec 12, 2007·Antimicrobial Agents and Chemotherapy·Sung Jae Shin, Michael T Collins
Sep 11, 2010·Expert Review of Clinical Immunology·Alka A Desai, Daniel J B Marks
Apr 10, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sung G KimDonald A Lein
Jul 3, 2016·Applied and Environmental Microbiology·Hannah B PooleyKarren M Plain
Jan 15, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·A LewinB Appel
Nov 2, 2014·Frontiers in Cellular and Infection Microbiology·Govardhan RathnaiahRaúl G Barletta
Oct 18, 2011·Applied and Environmental Microbiology·Natalia ElguezabalRamón A Juste
Jun 16, 2018·F1000Research·Ashwani KesarwaniPramod Upadhyay

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