A model for cystic endometrial hyperplasia/pyometra complex in the bitch

Theriogenology
N AroraP J Wright

Abstract

The objective of this study was to develop a reliable model for the study of the cystic endometrial hyperplasia and pyometra complex (CEH/P) in the bitch. Greyhound bitches (n = 15) were ovariectomised and allocated into three groups (Group 1, n = 5; Group 2, n = 5; Group 3, n = 10, including 5 used from Group 1). Simulated proestrus, estrus and diestrus were induced by treatment with estradiol benzoate and megestrol acetate. The duration of cervical opening during estrus was determined by the intra-vaginal infusion of radio-opaque medium and subsequent radiography of the uterus (Group 1). One milliliter of a culture of Escherichia coli (with five uro-pathogenic virulence factors as identified by PCR: pap, sfa, hlyA, cnf1 and fim) was inoculated intra-vaginally daily throughout the simulated estrus (Group 2). One milliliter of the culture (n = 6) or sterile Luria-Bertani broth (n = 4) was introduced directly into the uterus on simulated diestrus Days 8 or 12 (Group 3). Necropsies were performed 12 and 7-14 days after the inoculation (Groups 2 and 3). The cervix remained open throughout the duration of simulated estrus (5-6 days) in four out of five bitches, and for a shorter duration (3 days of a 6-day estrus period) in one bit...Continue Reading

References

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Citations

Jun 12, 2016·Reproductive Biology·Ewelina Jursza-Piotrowska, Marta J Siemieniuch
Apr 10, 2012·The Veterinary Clinics of North America. Small Animal Practice·Antonio Ortega-PachecoMatilde Jiménez-Coello
Jan 4, 2013·Reproduction in Domestic Animals = Zuchthygiene·N KrekelerP J Wright
Dec 8, 2014·Theriogenology·E JurszaM J Siemieniuch
May 21, 2015·Reproduction, Fertility, and Development·Christina D MarthNatali Krekeler
Nov 4, 2016·Reproduction in Domestic Animals = Zuchthygiene·R Hagman
Dec 9, 2008·Journal of Feline Medicine and Surgery·Abdulkadir KeskinAhmet Gumen
Jan 7, 2016·Journal of Feline Medicine and Surgery·Fiona Hollinshead, Natali Krekeler
Aug 2, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Murside Ayse DemirelAli Fuat Cicek
Jun 13, 2019·Reproduction in Domestic Animals = Zuchthygiene·Monica MelandriSalvatore Alonge
Aug 16, 2016·Journal of Veterinary Science·Daniel Maya-PulgarinJuan Guillermo Maldonado-Estrada
Nov 27, 2020·Journal of Feline Medicine and Surgery·Claudia BinderChristine Aurich

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