A model for normothermic preservation of the rat liver

Tissue Engineering
H TolboomMartin L Yarmush

Abstract

Current techniques for the preservation of donor livers typically rely on cold temperatures (approximately 0-4 degrees C) to slow down metabolic processes. Recently, normothermic extracorporeal liver perfusion (NELP) has regained interest as a potentially promising approach for long-term liver preservation. Unlike cold-storage techniques, NELP attempts to maintain the liver in a near physiological environment, thus enabling normal metabolic and tissue repair processes to take place, which may help in the recovery of ischemically damaged and fatty donor livers, both of which represent significant untapped sources of donor livers. However, NELP is technically more complex than cold-storage techniques, and the lack of standardized small animal models limits its development. Here we describe a rat NELP system that is simple and cost-effective to run. We show that rat livers that underwent NELP for 6 h could be routinely transplanted into syngeneic recipient rats with excellent 1-month survival. During perfusion, the release of cytosolic enzymes, bile and urea production, and oxygen uptake rate could be readily monitored, thus providing a comprehensive picture of hepatic function before transplantation. This system will help in the ...Continue Reading

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Citations

Jan 22, 2009·Transplantation·Herman TolboomMartin L Yarmush
Jun 27, 2012·BMC Research Notes·Sinem PerkKorkut Uygun
Mar 23, 2011·Annual Review of Biomedical Engineering·Stephen F BadylakKorkut Uygun
May 10, 2011·The Journal of Surgical Research·Herman TolboomMartin L Yarmush
Jun 11, 2009·Metabolic Engineering·Deepak NagrathMartin L Yarmush
Jul 1, 2008·Transplantation Proceedings·H TolboomM L Yarmush
Jul 5, 2008·The Journal of Surgical Research·François BerthiaumeMartin L Yarmush
Apr 23, 2015·Scientific Reports·Jun IshikawaTakashi Tsuji
Apr 18, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P DutkowskiP-A Clavien
Jul 15, 2011·Biotechnology and Bioengineering·Mehmet A OrmanFrancois Berthiaume
Apr 25, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·B G BruinsmaM-L Izamis
Jan 31, 2014·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Maria-Louisa IzamisMichalakis A Averkiou
Jul 16, 2014·Ultrasound in Medicine & Biology·Maria-Louisa IzamisMichalakis A Averkiou
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Aug 1, 2019·Tissue Engineering. Part a·Maximilian NösserNathanael Raschzok
Dec 12, 2018·Transplantation Direct·Isabel M A BrüggenwirthPaulo N Martins

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