A model for quantitative measurement of sulfur mustard skin lesions in the rabbit ear

Toxicology
A ZlotogorskiA Shafran

Abstract

The search for treatment and protection against the vesicant and inflammatory skin lesions induced by sulfur mustard suffers from the lack of a good in vivo reproducible model. We applied sulfur mustard (25-500 microg/cm2) to the outer surface of the ears of 10 rabbits and measured the edema formation 12, 24 and 48 h post-application with a caliper especially designed for soft matter. There was a dose-dependent linear increase in edema magnitude in the range from 25 to 150 microg/cm2. Maximal edema was observed after 12 h. There was a 12% reduction in edema size 24 h after application and a further decrease after 48 h. Skin thickness, inflammatory cell infiltrate, necrosis and vesiculation were evaluated in biopsies taken after 24 h. We found the same dose-related increase both in skin thickness and in degree of blister formation. This simple dose-response in vivo model can be used for evaluation of the dermal inflammation induced by topical application of sulfur mustard. This model has the additional advantage of a built-in control, namely the untreated contralateral ear. Consequently, this model can serve as a useful tool for future screening of potential compounds for prevention and treatment of sulfur mustard-induced skin l...Continue Reading

References

Aug 22, 1977·Archives of Dermatological Research·E G WeirichA H Kirkwood
Jul 1, 1991·The British Journal of Dermatology·A G QuinnP M Farr
Oct 1, 1990·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M M MershonJ V Wade
Nov 1, 1989·American Industrial Hygiene Association Journal·K SchoeneG Wodtke
Sep 1, 1993·Bulletin of Environmental Contamination and Toxicology·R S ChauhanR C Malhotra
Jul 1, 1993·Cell Biology and Toxicology·F M CowanW J Smith
Aug 1, 1996·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·V Manny-AframianS Dikstein

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Citations

Nov 18, 2000·Journal of Biochemical and Molecular Toxicology·C L SabourinR P Casillas
Dec 17, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Neera Tewari-SinghRajesh Agarwal
Jan 1, 2002·Toxicology Mechanisms and Methods·Dana R AndersonMark B Gold
Aug 10, 2007·Cutaneous and Ocular Toxicology·Myriane A IsidoreMadhusoodana P Nambiar
Apr 14, 2010·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Shlomit DachirTamar Kadar

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