A model for the self-organization of exit sites in the endoplasmic reticulum

Journal of Cell Science
Stephan HeinzerMatthias Weiss

Abstract

Exit sites (ES) are specialized domains of the endoplasmic reticulum (ER) at which cargo proteins of the secretory pathway are packaged into COPII-coated vesicles. Although the essential COPII proteins (Sar1p, Sec23p-Sec24p, Sec13p-Sec31p) have been characterized in detail and their sequential binding kinetics at ER membranes have been quantified, the basic processes that govern the self-assembly and spatial organization of ERES have remained elusive. Here, we have formulated a generic computational model that describes the process of formation of ERES on a mesoscopic scale. The model predicts that ERES are arranged in a quasi-crystalline pattern, while their size strongly depends on the cargo-modulated kinetics of COPII turnover - that is, a lack of cargo leads to smaller and more mobile ERES. These predictions are in favorable agreement with experimental data obtained by fluorescence microscopy. The model further suggests that cooperative binding of COPII components, for example mediated by regulatory proteins, is a key factor for the experimentally observed organism-specific ERES pattern. Moreover, the anterograde secretory flux is predicted to grow when the average size of ERES is increased, whereas an increase in the numbe...Continue Reading

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P G Saffman, M Delbrück
Oct 1, 1996·The Journal of Cell Biology·S I BannykhW E Balch
Dec 28, 1999·Cell·K J ZaalJ Lippincott-Schwartz
Sep 12, 2000·Molecular Biology of the Cell·A T Hammond, B S Glick
Jun 5, 2001·Nature Cell Biology·B AntonnyR Schekman
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Z LederkremerT Kirchhausen
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·K MatsuokaJ E Heuser
Jul 18, 2002·Traffic·Meir Aridor, Linton M Traub
Oct 3, 2002·Nature Cell Biology·Brooke J BevisBenjamin S Glick
Sep 23, 2003·EMBO Reports·Markus ElsnerMatthias Weiss
Mar 12, 2004·FEBS Letters·Pierrick DudognonJean-Pierre Paccaud
May 8, 2004·Trends in Cell Biology·Matthias Weiss, Tommy Nilsson
Jan 25, 2005·Nature Structural & Molecular Biology·Ken Sato, Akihiko Nakano
Aug 23, 2005·Current Biology : CB·Pamela L ConnerlyBenjamin S Glick
Jan 13, 2006·Nature·Scott M StaggWilliam E Balch
Jan 25, 2006·Current Biology : CB·Rebecca ForsterRainer Pepperkok
Jun 24, 2006·The EMBO Journal·Heiko RunzRainer Pepperkok
Jul 5, 2006·The Journal of Cell Biology·Yusong Guo, Adam D Linstedt
Jul 11, 2006·Biophysical Journal·Gernot Guigas, Matthias Weiss
Mar 18, 2008·Applied Optics·A Santos, I T Young

❮ Previous
Next ❯

Citations

Apr 12, 2011·Nature Cell Biology·Kristen WitteAnjon Audhya
Dec 24, 2011·Nature Cell Biology·Giulia ZanettiRandy Schekman
Dec 29, 2010·The Journal of General Physiology·Rahul SrinivasanHenry A Lester
Dec 8, 2009·Journal of Lipid Research·Shahzad SiddiqiCharles M Mansbach
Jul 30, 2009·Journal of Cell Science·Helen HughesDavid J Stephens
Sep 6, 2013·Molecular Biology of the Cell·Nike BharuchaBenjamin S Glick
Oct 1, 2013·Trends in Cell Biology·Rossella VendittiMaria Antonietta De Matteis
Nov 9, 2010·Molecular Membrane Biology·Katy E RoutledgeWilliam E Balch
Oct 24, 2009·FEBS Letters·Annika Budnik, David J Stephens
Jun 10, 2009·The Journal of Cell Biology·Ana M VacaruJoost C M Holthuis
Sep 19, 2009·The EMBO Journal·Kazuhito V TabataHiroyuki Noji
Nov 13, 2009·Current Biology : CB·Anna V ShnyrovaJoshua Zimmerberg
Dec 29, 2009·Biochimica Et Biophysica Acta·Elena S NadezhdinaPavel A Ivanov
Feb 7, 2019·Physiology·Magdalene K MontgomeryMatthew J Watt
Jun 1, 2012·Biophysics Reviews·Mária Hanulová, Matthias Weiss
Jun 20, 2018·Histochemistry and Cell Biology·Janine McCaughey, David J Stephens
Mar 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Julia HoffmannMatthias Weiss
Mar 13, 2021·Physical Review Letters·Marco ZamparoAndrea Gamba
Apr 30, 2009·Journal of Cell Science·Takayuki IinumaKatsuko Tani
Nov 4, 2010·Journal of Cell Science·Helen Hughes, David J Stephens
Dec 21, 2014·Journal of Cell Science·Kerstin D TillmannHesso Farhan
May 2, 2021·Biophysical Journal·Konstantin SpecknerMatthias Weiss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.